Modulation of Caspase 3 Activity by Cytokines
| Culture Conditions . | Caspase 3 Activity (U/2 × 105cells) . | ||||||
|---|---|---|---|---|---|---|---|
| DOX (0.5 μg/mL) . | CD40L L Cells . | Cytokines . | Daudi . | Raji . | BJAB . | BL36 . | BL70 . |
| − | − | — | 1.38 | 1.48 | 1.3 | 5.82 | 2.25 |
| + | − | — | 5.2 | 14.8 | 9.3 | 8.6 | 6.4 |
| + | + | — | 3.2* | 7.9* | 4.3* | 5.9* | 0.9* |
| + | − | IL-10 | 3.3* | 8.9* | 7.5* | 6.9 | 9.2 |
| + | − | IL-2 | 3.9* | 6.4* | 6.9* | 7.9 | 5.3 |
| + | − | IL-6 | 3.2* | 10.0* | 7.0* | 6.2 | 5.2 |
| + | − | TNF | 3.2* | 10.9* | 4.7* | 6.8 | 3.6 |
| + | + | IL-10 | 1.1† | 6.5 | 5.5 | 6.7 | 0.8 |
| + | + | IL-2 | 2.8 | 6.9 | 6.1 | 6.7 | 0.6 |
| + | + | IL-6 | 2.3† | 6.8 | 5.5 | 5.3 | 0.6 |
| + | + | TNF | 2.7 | 9.0 | 6.0 | 6.5 | 2.5 |
| Culture Conditions . | Caspase 3 Activity (U/2 × 105cells) . | ||||||
|---|---|---|---|---|---|---|---|
| DOX (0.5 μg/mL) . | CD40L L Cells . | Cytokines . | Daudi . | Raji . | BJAB . | BL36 . | BL70 . |
| − | − | — | 1.38 | 1.48 | 1.3 | 5.82 | 2.25 |
| + | − | — | 5.2 | 14.8 | 9.3 | 8.6 | 6.4 |
| + | + | — | 3.2* | 7.9* | 4.3* | 5.9* | 0.9* |
| + | − | IL-10 | 3.3* | 8.9* | 7.5* | 6.9 | 9.2 |
| + | − | IL-2 | 3.9* | 6.4* | 6.9* | 7.9 | 5.3 |
| + | − | IL-6 | 3.2* | 10.0* | 7.0* | 6.2 | 5.2 |
| + | − | TNF | 3.2* | 10.9* | 4.7* | 6.8 | 3.6 |
| + | + | IL-10 | 1.1† | 6.5 | 5.5 | 6.7 | 0.8 |
| + | + | IL-2 | 2.8 | 6.9 | 6.1 | 6.7 | 0.6 |
| + | + | IL-6 | 2.3† | 6.8 | 5.5 | 5.3 | 0.6 |
| + | + | TNF | 2.7 | 9.0 | 6.0 | 6.5 | 2.5 |
*P < .05 (Student’s paired t-test) as compared with DOX-treated cells in 6 experiments.
P < .05 (Student’s paired t-test) as compared with DOX-treated cells cocultured with CD40L L cells.