Effect of Various Plasmas Positive for Antiphospholipid-Related Markers on the AP C-Mediated Factor Va Proteolysis (aPC-FV) Using the Modified aPC-Resistance Test
| . | aPC-FV Ratio . | ||
|---|---|---|---|
| Patient Plasmas . | Mixing Studies 1:1 . | ||
| NP . | NP-β2neg . | ||
| Control plasmas (n = 200) | |||
| Whole plasmas | 2.54 (2.08-3.36) | 2.55 (2.19-2.96) | 2.50 (2.10-2.92) |
| NP (pooled control plasmas) | 2.55 | 2.55 | 2.51 |
| Isolated LA (n = 10) | |||
| Whole plasmas | 2.64 (2.19-3.24) | 2.59 (2.23-2.85) | |
| IgG-depleted plasmas | 2.57 (2.11-3.18) | 2.55 (2.28-2.80) | |
| Isolated aCLAb (n = 10) | |||
| Whole plasmas | 2.72 (2.31-3.30) | 2.63 (2.35-2.96) | |
| IgG-depleted plasmas | 2.68 (2.35-3.22) | 2.60 (2.38-3.01) | |
| Isolated aβ2GP1Ab (n = 10) | |||
| Whole plasmas | 1.52 (1.11-2.02) [1] | 1.75 (1.23-2.19) [3] | 1.93 (1.54-2.52) [5] |
| IgG-depleted plasmas | 2.42 (2.12-2.65) [2] | 2.45 (2.23-2.60) [4] | 2.47 (2.20-2.71) [6] |
| Isolated aFIIAb (n = 2) | |||
| Whole plasmas | (2.76-3.15) | (2.65-2.96) | |
| IgG-depleted plasmas | (2.80-3.04) | (2.71-2.82) | |
| Isolated aAnVAb (n = 10) | |||
| Whole plasmas | 2.61 (2.25-3.00) | 2.58 (2.36-2.88) | |
| IgG-depleted plasmas | 2.58 (2.29-3.05) | 2.55 (2.34-2.79) | |
| . | aPC-FV Ratio . | ||
|---|---|---|---|
| Patient Plasmas . | Mixing Studies 1:1 . | ||
| NP . | NP-β2neg . | ||
| Control plasmas (n = 200) | |||
| Whole plasmas | 2.54 (2.08-3.36) | 2.55 (2.19-2.96) | 2.50 (2.10-2.92) |
| NP (pooled control plasmas) | 2.55 | 2.55 | 2.51 |
| Isolated LA (n = 10) | |||
| Whole plasmas | 2.64 (2.19-3.24) | 2.59 (2.23-2.85) | |
| IgG-depleted plasmas | 2.57 (2.11-3.18) | 2.55 (2.28-2.80) | |
| Isolated aCLAb (n = 10) | |||
| Whole plasmas | 2.72 (2.31-3.30) | 2.63 (2.35-2.96) | |
| IgG-depleted plasmas | 2.68 (2.35-3.22) | 2.60 (2.38-3.01) | |
| Isolated aβ2GP1Ab (n = 10) | |||
| Whole plasmas | 1.52 (1.11-2.02) [1] | 1.75 (1.23-2.19) [3] | 1.93 (1.54-2.52) [5] |
| IgG-depleted plasmas | 2.42 (2.12-2.65) [2] | 2.45 (2.23-2.60) [4] | 2.47 (2.20-2.71) [6] |
| Isolated aFIIAb (n = 2) | |||
| Whole plasmas | (2.76-3.15) | (2.65-2.96) | |
| IgG-depleted plasmas | (2.80-3.04) | (2.71-2.82) | |
| Isolated aAnVAb (n = 10) | |||
| Whole plasmas | 2.61 (2.25-3.00) | 2.58 (2.36-2.88) | |
| IgG-depleted plasmas | 2.58 (2.29-3.05) | 2.55 (2.34-2.79) | |
Results are expressed as aPC-FV ratios (time obtained in the presence of aPC divided by the time obtained in absence of aPC) and are given as median value (range). Statistical analysis (Wilcoxon paired t-test): [1] v [2], [3] v [4], and [5]v [6]: P = .005; [1] v [3], [3] v [5], and [1] v [5]: P = .005. All other comparisons were nonsignificant.
Abbreviations: NP, normal plasma; NP-β2neg, normal plasma after β2-glycoprotein I depletion; LA, positive lupus anticoagulant; aCLAb, positive anticardiolipin IgG antibodies; aβ2GP1Ab, positive anti–β2-glycoprotein I IgG antibodies; aFIIAb, positive antiprothrombin IgG antibodies; aAnVAb, anti-annexin V IgG antibodies.