Dose-Response Kinetics of CD40L Effects on L3055 Cells
| CD40L Added (μg/mL) . | Induction of Homotypic Adhesions-150 . | Induction of CD23 Expression (MFI)-151 . | % Rescue From Anti-IgM-152 . |
|---|---|---|---|
| 5 | +++ | 72 (16) | 90 (21) |
| 1 | +++ | 70 (21) | 107 (14) |
| 0.2 | ++(+) | 23 (6) | 43 (17) |
| 0.04 | ++ | 7 (3) | 5 (2) |
| 0.008 | + | 5 (2) | 2 (1) |
| 0 | − | 6 (2) | 0 |
| CD40L Added (μg/mL) . | Induction of Homotypic Adhesions-150 . | Induction of CD23 Expression (MFI)-151 . | % Rescue From Anti-IgM-152 . |
|---|---|---|---|
| 5 | +++ | 72 (16) | 90 (21) |
| 1 | +++ | 70 (21) | 107 (14) |
| 0.2 | ++(+) | 23 (6) | 43 (17) |
| 0.04 | ++ | 7 (3) | 5 (2) |
| 0.008 | + | 5 (2) | 2 (1) |
| 0 | − | 6 (2) | 0 |
L3055 cells were placed in culture for the assays as described in Materials and Methods.
The presence of homotypic cell clustering was determined after 72 hours of culture by light microscopy using the following assessment criteria: +++, >50 cells per cluster; ++, >25 cells; +, >10; +/−, 2 to 10 cells. The results shown were seen in three separate experiments.
CD23 expression (in the presence of IL-4 at 20 ng/mL) was determined by FACS analysis at 72 hours and is given as the mean fluorescent intensity (MFI) of staining, with background (control) levels of staining with an irrelevant MoAb subtracted. The results are given as mean determinations from three experiments with SD in parentheses.