Effect of Treatment With RA on Drug-Stimulated DNA Cleavable Complex Formation and Apoptosis in HL-60 Cells
| Drug Treatment* . | Control . | RA Treated . | P† . |
|---|---|---|---|
| 0.5 μmol/L CPT | |||
| DNA C.C. | 2.6 ± 0.8‡ | 3.1 ± 1.0‡ | .6 |
| % Apoptosis | ND | ND | |
| 1 μmol/L CPT | |||
| DNA C.C. | 3.8 ± 0.4‡ | 4.4 ± 0.5‡ | .19 |
| % Apoptosis | 2.6 ± 0.31-153 | 1.7 ± 0.21-153 | .003 |
| 5 μmol/L CPT | |||
| DNA C.C. | 9.3 ± 0.8 | 8.2 ± 1.1 | .22 |
| % Apoptosis | 20.2 ± 3.5 | 11.6 ± 3.7 | .006 |
| 10 μmol/L CPT | |||
| DNA C.C. | 12.7 ± 2.4 | 9.2 ± 2.0 | .13 |
| % Apoptosis | 27.4 ± 3.7 | 16.5 ± 3.6 | <.001 |
| 1 μmol/L VP-16 | |||
| DNA C.C. | 2.6 ± 0.4 | 2.2 ± 0.4 | .27 |
| % Apoptosis | 1.0 ± 0.5 | 1.7 ± 1.2 | .4 |
| 10 μmol/L VP-16 | |||
| DNA C.C. | 8.8 ± 3.7 | 4.9 ± 0.9 | .15 |
| % Apoptosis | 10.5 ± 3.7 | 4.7 ± 1.5 | .012 |
| Drug Treatment* . | Control . | RA Treated . | P† . |
|---|---|---|---|
| 0.5 μmol/L CPT | |||
| DNA C.C. | 2.6 ± 0.8‡ | 3.1 ± 1.0‡ | .6 |
| % Apoptosis | ND | ND | |
| 1 μmol/L CPT | |||
| DNA C.C. | 3.8 ± 0.4‡ | 4.4 ± 0.5‡ | .19 |
| % Apoptosis | 2.6 ± 0.31-153 | 1.7 ± 0.21-153 | .003 |
| 5 μmol/L CPT | |||
| DNA C.C. | 9.3 ± 0.8 | 8.2 ± 1.1 | .22 |
| % Apoptosis | 20.2 ± 3.5 | 11.6 ± 3.7 | .006 |
| 10 μmol/L CPT | |||
| DNA C.C. | 12.7 ± 2.4 | 9.2 ± 2.0 | .13 |
| % Apoptosis | 27.4 ± 3.7 | 16.5 ± 3.6 | <.001 |
| 1 μmol/L VP-16 | |||
| DNA C.C. | 2.6 ± 0.4 | 2.2 ± 0.4 | .27 |
| % Apoptosis | 1.0 ± 0.5 | 1.7 ± 1.2 | .4 |
| 10 μmol/L VP-16 | |||
| DNA C.C. | 8.8 ± 3.7 | 4.9 ± 0.9 | .15 |
| % Apoptosis | 10.5 ± 3.7 | 4.7 ± 1.5 | .012 |
Control and RA-treated (96 hours) HL-60 cells were evaluated for drug-stimulated DNA cleavable complex (DNA C.C.) formation by the SDS-KCl technique25 and apoptosis by the TUNEL assay using fluorescein-12-dUTP.30
Abbreviation: ND, not determined.
Control and RA-treated (96 hours) HL-60 cells were incubated at 37°C with the indicated concentrations of CPT (TOPO I poison) or VP-16 (TOPO II poison) for 1 hour.
t-test P values of differences in DNA C.C. and the percentage of apoptosis between control and RA-treated groups.
Fold-increase in drug-stimulated DNA cleavable complex formation compared to the untreated control. Values are the mean ± standard deviation from at least triplicate experiments.
% Apoptosis. Values are mean ± standard deviation from at least triplicate experiments.