Rate Constants for Heparin Pentasaccharide Binding to Antithrombin Variants
| . | Ionic Strength . | K1 (μmol/L) . | k2 (s−1) . | kon(μmol/L−1 · s−1) . | koff (k−2) (s−1) . |
|---|---|---|---|---|---|
| α-Antithrombin | 0.15 | 20.8 ± 2.1 | 563 ± 35.4 | 38.7 ± 1.6 | 2.1* |
| 0.3 | 11.6 ± 0.4 | 6.6 ± 0.5 | |||
| Thr85Met | 0.15 | 34.2 ± 3.5 | 2172 ± 213 | 64.3 ± 3.6 | 0.06* |
| 0.3 | 22.6 ± 0.8 | 3.7 ± 0.6 | |||
| Rouen VI | 0.15 | 54.9 ± 4.96 | 1464 ± 145 | 48.2 ± 4.1 | 0.62* |
| 0.3 | 14.96 ± 0.22 | 3.64 ± 0.46 |
| . | Ionic Strength . | K1 (μmol/L) . | k2 (s−1) . | kon(μmol/L−1 · s−1) . | koff (k−2) (s−1) . |
|---|---|---|---|---|---|
| α-Antithrombin | 0.15 | 20.8 ± 2.1 | 563 ± 35.4 | 38.7 ± 1.6 | 2.1* |
| 0.3 | 11.6 ± 0.4 | 6.6 ± 0.5 | |||
| Thr85Met | 0.15 | 34.2 ± 3.5 | 2172 ± 213 | 64.3 ± 3.6 | 0.06* |
| 0.3 | 22.6 ± 0.8 | 3.7 ± 0.6 | |||
| Rouen VI | 0.15 | 54.9 ± 4.96 | 1464 ± 145 | 48.2 ± 4.1 | 0.62* |
| 0.3 | 14.96 ± 0.22 | 3.64 ± 0.46 |
*Calculated from Kd and konvalues.