Cloning Efficiency of M1, M1neo, and M1Egr-1 Cells
| Cell Type . | No. of Colonies . | ||
|---|---|---|---|
| Untreated . | IL-6 (ng/mL) . | ||
| 1 . | 5 . | ||
| M1 | 197 | 120 | 16 |
| M1neo (11) | 199 | 116 | 28 |
| M1neo (12) | 202 | 124 | 22 |
| M1Egr-1 (2) | 72 | 36 | 2 |
| M1Egr-1 (3) | 64 | 30 | 7 |
| M1Egr-1 (4) | 90 | 60 | 5 |
| M1Egr-1 (5) | 69 | 34 | 6 |
| Cell Type . | No. of Colonies . | ||
|---|---|---|---|
| Untreated . | IL-6 (ng/mL) . | ||
| 1 . | 5 . | ||
| M1 | 197 | 120 | 16 |
| M1neo (11) | 199 | 116 | 28 |
| M1neo (12) | 202 | 124 | 22 |
| M1Egr-1 (2) | 72 | 36 | 2 |
| M1Egr-1 (3) | 64 | 30 | 7 |
| M1Egr-1 (4) | 90 | 60 | 5 |
| M1Egr-1 (5) | 69 | 34 | 6 |
Colonies in soft agar were counted 14 days after seeding 300 cells per 60-mm dish. All experiments were initiated with nonadherent cells. Numbers represent the mean of three independent experiments with standard deviations up to ±15%.