Table 1.

Differentiation-Associated Properties of M1, M1neo, and M1Egr-1 Cells

Cell Lines IL-6 (ng/mL) BlastCells Types (%)*% Adhered Cells*Receptors (%)*
Inter. Mature Fc C3
M1  0  99  <1  0  0  <2  <1.5  
 1 95  5  0  10  12  8  
 50  0  32  68 62  52  74  
M1neo.11  0  98  <2  0  0 <2  1  
 1  94  6  0  9  13  9 
 50  0  29  71  61  49  70  
M1neo.12  0 98  2  0  0  2  <2  
 1  96  4  0 8  11  7  
 50  0  33  67  60  48  69 
M1Egr.1.2  0  7  54  39  57  35  39  
 1 4  27  69  65  58  64  
 50  0  18  82 90  68  78  
M1Egr.1.3  0  9  58  33  52 28  32  
 1  5  30  65  60  56  63 
 50  0  22  78  82  66  77 
Cell Lines IL-6 (ng/mL) BlastCells Types (%)*% Adhered Cells*Receptors (%)*
Inter. Mature Fc C3
M1  0  99  <1  0  0  <2  <1.5  
 1 95  5  0  10  12  8  
 50  0  32  68 62  52  74  
M1neo.11  0  98  <2  0  0 <2  1  
 1  94  6  0  9  13  9 
 50  0  29  71  61  49  70  
M1neo.12  0 98  2  0  0  2  <2  
 1  96  4  0 8  11  7  
 50  0  33  67  60  48  69 
M1Egr.1.2  0  7  54  39  57  35  39  
 1 4  27  69  65  58  64  
 50  0  18  82 90  68  78  
M1Egr.1.3  0  9  58  33  52 28  32  
 1  5  30  65  60  56  63 
 50  0  22  78  82  66  77 

*Determined 3 days after the cells were seeded at a density of 0.15 × 106 cells/mL. For details of analysis of differentiated phenotypes, receptor assays and determination of percentage of cell adherence, see Materials and Methods. All experiments were initiated with nonadherent cells. Four M1Egr1 clones and four M1neo clones were used in these experiments. All clones of the same type gave similar results and data are shown for two clones. All values represent the mean of three independent determinations with standard deviations up to 12% (ie, 10% ± 1.2%).

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