Table 2.

IFN-γ Production by Human PBMC and B Cells After 48 Hours Stimulation in the Presence of Retinoids

Experiment 1 Experiment 2 Experiment 3Experiment 4 Experiment 5 Experiment 6
Control  145  64  10  3.2  4  7  
CD40 + IL-4 123  61  5.6  3.3  <3  <3 
CD40 + IL-4  + all-trans RA  117  44  5.6 4.0  <3  <3  
CD40 + IL-4   + 13-cis RA 35  20  5.2  5.3  <3  <3 
Experiment 1 Experiment 2 Experiment 3Experiment 4 Experiment 5 Experiment 6
Control  145  64  10  3.2  4  7  
CD40 + IL-4 123  61  5.6  3.3  <3  <3 
CD40 + IL-4  + all-trans RA  117  44  5.6 4.0  <3  <3  
CD40 + IL-4   + 13-cis RA 35  20  5.2  5.3  <3  <3 

Production of secreted INF-γ protein (pg/mL) in CD40 + IL-4 activated PBMC (exps 1-4) and B cells (exps 5-6) from normal donors. IFNγ was measured in the supernatants of 106/mL cells by ELISA after 48 hours stimulation with anti-CD40 (1 μg/mL) and IL-4 (5 ng/mL) and/or all-trans or 13-cis RA (10−10 mol/L). Values are shown as means of duplicates.

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