Table 1. Effects of all- trans and 13-... | American Society of Hematology

Table 1.

Effects of all-trans and 13-cis Retinoic Acid on IgA, IgG, and IgE Production in Anti-CD40 + IL-4–Stimulated IgM⁺ Peripheral B Cells

	IgA (ng/mL)	IgG (ng/mL)	IgE (pg/mL)
Medium	<100	<100	<100
Anti-CD40 + IL-4	398 ± 22	510 ± 34	2040 ± 126
Anti-CD40 + IL-4 + R1^10-14 mol/L	401 ± 43	480 ± 45	1884 ± 110
Anti-CD40 + IL-4 + R1^10-12 mol/L	406 ± 35	490 ± 38	992 ± 150
Anti-CD40 + IL-4 + R1^10-10 mol/L	402 ± 37	508 ± 47	798 ± 123
Anti-CD40 + IL-4 + R1^10-8 mol/L	402 ± 47	510 ± 42	289 ± 105
Anti-CD40 + IL-4 + R2^10-14 mol/L	400 ± 46	496 ± 38	1773 ± 136
Anti-CD40 + IL-4 + R2^10-12 mol/L	409 ± 37	489 ± 45	1649 ± 156
Anti-CD40 + IL-4 + R2^10-10 mol/L	406 ± 43	498 ± 38	737 ± 134
Anti-CD40 + IL-4 + R2^10-8 mol/L	403 ± 27	491 ± 42	261 ± 128

	IgA (ng/mL)	IgG (ng/mL)	IgE (pg/mL)
Medium	<100	<100	<100
Anti-CD40 + IL-4	398 ± 22	510 ± 34	2040 ± 126
Anti-CD40 + IL-4 + R1^10-14 mol/L	401 ± 43	480 ± 45	1884 ± 110
Anti-CD40 + IL-4 + R1^10-12 mol/L	406 ± 35	490 ± 38	992 ± 150
Anti-CD40 + IL-4 + R1^10-10 mol/L	402 ± 37	508 ± 47	798 ± 123
Anti-CD40 + IL-4 + R1^10-8 mol/L	402 ± 47	510 ± 42	289 ± 105
Anti-CD40 + IL-4 + R2^10-14 mol/L	400 ± 46	496 ± 38	1773 ± 136
Anti-CD40 + IL-4 + R2^10-12 mol/L	409 ± 37	489 ± 45	1649 ± 156
Anti-CD40 + IL-4 + R2^10-10 mol/L	406 ± 43	498 ± 38	737 ± 134
Anti-CD40 + IL-4 + R2^10-8 mol/L	403 ± 27	491 ± 42	261 ± 128

IgM⁺ B cells (10⁶/mL) were incubated for 10 days in the presence of CD40 + IL-4 (1 μg/mL and 5 ng/mL) alone or in combination with all-trans RA (R1) or 13-cis RA (R2). Igs were detected in the supernatants by ELISA, values are shown as means of triplicates. Shown is one representative of four experiments.

View Large

This Feature Is Available To Subscribers Only