Effect of NO Donors on the Stimulation of NK and LAK Activity by IL-12 and TNF
| Culture Conditions . | % of Cell Lysis . | |
|---|---|---|
| NK Activity . | LAK Activity . | |
| Medium | 15 ± 4.5 | 3 ± 2.3 |
| IL-12 + TNFα | 61 ± 9.3 | 22 ± 7.5 |
| IL-12 + TNFα + SNP (10 μmol/L) | 59 ± 2.3 | 15 ± 5.4 |
| IL-12 + TNFα + SNP (20 μmol/L) | 46 ± 2.1 | 7 ± 3.2 |
| IL-12 + TNFα + SNP (50 μmol/L) | 32 ± 3.0 | 6 ± 1.2 |
| Medium | 31 ± 1.05 | ND |
| IL-12 + TNFα | 65 ± 3.01 | 47 ± 1.5 |
| IL-12 + TNFα + SIN-1 (10 μM) | 52 ± 1.1 | 27 ± 0.6 |
| IL-12 + TNFα + SIN-1 (20 μM) | 32 ± 2.0 | 15 ± 2.6 |
| IL-12 + TNFα + SIN-1 (50 μM) | 28 ± 0.7 | 10 ± 0.2 |
| Culture Conditions . | % of Cell Lysis . | |
|---|---|---|
| NK Activity . | LAK Activity . | |
| Medium | 15 ± 4.5 | 3 ± 2.3 |
| IL-12 + TNFα | 61 ± 9.3 | 22 ± 7.5 |
| IL-12 + TNFα + SNP (10 μmol/L) | 59 ± 2.3 | 15 ± 5.4 |
| IL-12 + TNFα + SNP (20 μmol/L) | 46 ± 2.1 | 7 ± 3.2 |
| IL-12 + TNFα + SNP (50 μmol/L) | 32 ± 3.0 | 6 ± 1.2 |
| Medium | 31 ± 1.05 | ND |
| IL-12 + TNFα | 65 ± 3.01 | 47 ± 1.5 |
| IL-12 + TNFα + SIN-1 (10 μM) | 52 ± 1.1 | 27 ± 0.6 |
| IL-12 + TNFα + SIN-1 (20 μM) | 32 ± 2.0 | 15 ± 2.6 |
| IL-12 + TNFα + SIN-1 (50 μM) | 28 ± 0.7 | 10 ± 0.2 |
Purified NK cells were stimulated for 3 days with IL-12 + TNFα and in the presence or not of various concentrations of the NO donors SNP or SIN-1. At the end of the incubation, the effector cells were washed and tested for their cytotoxic activity on NK (K562 at an E/T ratio 12.5:1) (left column) and LAK (Daudi at an E/T ratio 25:1) (right column) target cells. Results are expressed as the mean ± SD of triplicate samples of one representative experiment of five (Sin-1) or three (SNP) performed.
Abbreviation: ND, not determined.