Table 1. Summary of Biological and... | American Society of Hematology

Table 1.

Summary of Biological and Biochemical Activities Conferred by Constitutively Active hβc Mutants

Mutant^-150	Location	Proliferative Activity in BAF-B03 Cells		Tyrosine Phosphorylation of hβc^-151		MAP Kinase Activation^-151	STAT5 Activation^-151	JAK2 Activation^-151
Mutant^-150	Location	Alone	+hGMRα and hGM-CSF	Alone	+hGMRα and hGM-CSF	MAP Kinase Activation^-151	STAT5 Activation^-151	JAK2 Activation^-151
L356P	Domain 4, β-strand B	No	No	No	No	Yes	Yes	Yes
W358N	Domain 4, β-strand B	No	No	No	No	Yes	Yes	ND
I374N	Domain 4, β-strand C	No	Yes	No	Yes	Yes	Yes	Yes
Q375P	Domain 4, β-strand C	No	Yes	No	Yes	Yes	Yes	ND
Y376N	Domain 4, β-strand C	No	Yes	No	Yes	Yes	Yes	ND
W383R	Domain 4, β-strand D	No	Yes	No	Yes	Yes	Yes	ND
L399P	Domain 4, β-strand E	No	Yes	No	No	Yes	Yes	Yes
L445Q	Transmembrane domain	No	Yes	No	Yes	Yes	Yes	ND
V449E	Transmembrane domain	Yes	ND	Yes	Yes	Yes	Yes	Yes
A459D	Transmembrane domain	Yes	ND	Yes	Yes	Yes	Yes	ND
R461C	Cytoplasmic domain	No	Yes	Yes	Yes	Yes	Yes	ND
H544R	Cytoplasmic domain, box 2	No	Yes	No	No	Yes	Yes	Yes

Mutant^-150	Location	Proliferative Activity in BAF-B03 Cells		Tyrosine Phosphorylation of hβc^-151		MAP Kinase Activation^-151	STAT5 Activation^-151	JAK2 Activation^-151
Mutant^-150	Location	Alone	+hGMRα and hGM-CSF	Alone	+hGMRα and hGM-CSF	MAP Kinase Activation^-151	STAT5 Activation^-151	JAK2 Activation^-151
L356P	Domain 4, β-strand B	No	No	No	No	Yes	Yes	Yes
W358N	Domain 4, β-strand B	No	No	No	No	Yes	Yes	ND
I374N	Domain 4, β-strand C	No	Yes	No	Yes	Yes	Yes	Yes
Q375P	Domain 4, β-strand C	No	Yes	No	Yes	Yes	Yes	ND
Y376N	Domain 4, β-strand C	No	Yes	No	Yes	Yes	Yes	ND
W383R	Domain 4, β-strand D	No	Yes	No	Yes	Yes	Yes	ND
L399P	Domain 4, β-strand E	No	Yes	No	No	Yes	Yes	Yes
L445Q	Transmembrane domain	No	Yes	No	Yes	Yes	Yes	ND
V449E	Transmembrane domain	Yes	ND	Yes	Yes	Yes	Yes	Yes
A459D	Transmembrane domain	Yes	ND	Yes	Yes	Yes	Yes	ND
R461C	Cytoplasmic domain	No	Yes	Yes	Yes	Yes	Yes	ND
H544R	Cytoplasmic domain, box 2	No	Yes	No	No	Yes	Yes	Yes

Abbreviation: ND, not determined.

All mutants confer factor-independent proliferation on FDC-P1 cells. Mutants isolated in previous studies are underlined.

Signaling analyses were performed on factor-independent FDC-P1 cells (see Figs 3, 4, 5, and 6).