A separate experiment showed that immunodepletion of gp120 with a gp120-specific MoAb removed the stimulating effect on sCD14-release from monocytes. Adherent monocytes from healthy donors were treated for 72 hours with gp120-immunodepleted solution and compared with equal amounts of liquid from the control gp120 solution (without gp120 MoAb in immunodepletion procedure) in sCD14-releasing ability. Numbers are the mean of duplicates. The concentration of sCD14 in unstimulated culture was 49 ng/mL.