Cell-Autonomous Lack of Proplatelet Formation
| Source of Cultured Megakaryocytes . | Source of Conditioned Medium . | ||
|---|---|---|---|
| +/+ . | +/− . | −/− . | |
| +/+ | +++ | +++ | +++ |
| +/− | ND | ND | +++ |
| −/− | − | − | − |
| Source of Adherent (stromal) Cells | |||
| +/+ | +/− | −/− | |
| +/+ | +++ | ND | +++ |
| +/− | +++ | ND | +++ |
| −/− | − | ND | − |
| Source of Cultured Megakaryocytes . | Source of Conditioned Medium . | ||
|---|---|---|---|
| +/+ . | +/− . | −/− . | |
| +/+ | +++ | +++ | +++ |
| +/− | ND | ND | +++ |
| −/− | − | − | − |
| Source of Adherent (stromal) Cells | |||
| +/+ | +/− | −/− | |
| +/+ | +++ | ND | +++ |
| +/− | +++ | ND | +++ |
| −/− | − | ND | − |
Proplatelet formation by cultured murine megakaryocytes of different p45 NF-E2 genotypes (+/+, +/−, or −/−) when culture medium was replaced by conditioned medium from the indicated cultures (top panel) or nonadherent cells (including megakaryocytes) were transferred to an adherent cell monolayer established from fetal livers of the indicated genotype (bottom panel). Neither quantitative nor qualitative differences in proplatelet formation (represented here as abundant [+++] or absent [−]) were observed over 1 week of observation between these and unmanipulated cultures in two separate experiments for each type.
Abbreviation: ND, not done.