Blood Clearance Half-Life of the Free Anti-Id Abs and the Anti-Id–IL-2 Fusion Proteins
| Ab . | t1/2 . | |
|---|---|---|
| α . | β . | |
| S5A8 | 5.6 h | 80.2 h |
| chS5A8 | 3.2 h | 90.5 h |
| chS5A8–IL-2 | 2.4 h | 17.6 h |
| scFvS5A8–IL-2 | 5 min | 49 min |
| Ab . | t1/2 . | |
|---|---|---|
| α . | β . | |
| S5A8 | 5.6 h | 80.2 h |
| chS5A8 | 3.2 h | 90.5 h |
| chS5A8–IL-2 | 2.4 h | 17.6 h |
| scFvS5A8–IL-2 | 5 min | 49 min |
BALB/c mice were injected IV with 25 μg of the various anti-Id Abs and blood samples were collected at various time points after injection. The specific Ab concentration in each sample was determined by ELISA for anti-Id determinant. The α phase (distribution) and the β phase (elimination) half-life values were determined as described in Materials and Methods.