Hematopoietic Stem Cell Numbers at 20 Weeks After Completion of a Six-Cycle Administration of Cytotoxic Agents. Effect of G-CSF
| Treatment . | CAFC-35 Per Hind Limb . | P Value4-150,4-151 . | RU Per Hind Limb . | P Value4-150 . | ||
|---|---|---|---|---|---|---|
| No G-CSF . | +G-CSF . | No G-CSF . | +G-CSF . | |||
| Saline | 674 (500-908) | 263 (141-489) | <.05 | 438 ± 90 (12) | 296 ± 94 (10) | .29 |
| VP-16 + cisplatinum | NA | NA | 426 ± 76 (6) | 316 ± 132 (6) | .49 | |
| Cyclophosphamide | 362 (199-660) | 320 (171-599) | NS | 610 ± 121 (14) | 666 ± 183 (12) | .80 |
| Carboplatinum | 290 (188-285) | 144 (103-200) | NS | 244 ± 79 (10) | 145 ± 48 (11) | .30 |
| Chlorambucil | 371 (278-496) | 185 (120-285) | NS | 135 ± 12 (6) | 60 ± 11 (4) | <.01 |
| BCNU | 222 (134-368) | 133 (49-359) | NS | 122 ± 13 (19) | 13.2 ± 2.8 (20) | <.01 |
| TBI | 298 (173-512) | 55 (32-97) | <.05 | 31 ± 8 (8) | 4.5 ± 1.1 (4) | .01 |
| Treatment . | CAFC-35 Per Hind Limb . | P Value4-150,4-151 . | RU Per Hind Limb . | P Value4-150 . | ||
|---|---|---|---|---|---|---|
| No G-CSF . | +G-CSF . | No G-CSF . | +G-CSF . | |||
| Saline | 674 (500-908) | 263 (141-489) | <.05 | 438 ± 90 (12) | 296 ± 94 (10) | .29 |
| VP-16 + cisplatinum | NA | NA | 426 ± 76 (6) | 316 ± 132 (6) | .49 | |
| Cyclophosphamide | 362 (199-660) | 320 (171-599) | NS | 610 ± 121 (14) | 666 ± 183 (12) | .80 |
| Carboplatinum | 290 (188-285) | 144 (103-200) | NS | 244 ± 79 (10) | 145 ± 48 (11) | .30 |
| Chlorambucil | 371 (278-496) | 185 (120-285) | NS | 135 ± 12 (6) | 60 ± 11 (4) | <.01 |
| BCNU | 222 (134-368) | 133 (49-359) | NS | 122 ± 13 (19) | 13.2 ± 2.8 (20) | <.01 |
| TBI | 298 (173-512) | 55 (32-97) | <.05 | 31 ± 8 (8) | 4.5 ± 1.1 (4) | .01 |
CAFC frequencies were determined from pooled marrow from 3 to 5 mice per experiment, the average frequency was calculated from 1 to 3 experiments with 95% confidence intervals (given in parentheses). RU/hind limb was calculated from 4 to 20 recipients (no. given in parentheses) that had received marrow pooled from 3 to 5 treated mice; 1 to 3 separate competitive repopulation assays were performed (see Materials and Methods for details).
Abbreviations: NA, not available; NS, not significant.
P values are for comparison with drug alone.
P values for CAFC-35 are shown as <.05 (.01 < P < .05), <.01 (.001 < P < .01) or as P < .001.