Coculture of CD34+CD38+/−Cells With AGM-S3 Cells
| Duration of Coculture . | Cultured Cells . | No. of Progenitors/Well . | |||
|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Mix . | Total . | ||
| 0 | CD34+CD38+ | 150 ± 25 | 105 ± 15 | 50 ± 5 | 305 ± 40 |
| CD34+CD38− | 25 ± 5 | 10 ± 3 | 30 ± 6 | 65 ± 8 | |
| 4 wk | CD34+CD38+ | 560 ± 40 | 40 ± 5 | 386 ± 45 | 986 ± 90 |
| CD34+CD38− | 120 ± 20 | 20 ± 5 | 136 ± 20 | 276 ± 56 | |
| 6 wk | CD34+CD38+ | 210 ± 40 | 0 | 0 | 210 ± 40 |
| CD34+CD38− | 310 ± 30 | 30 ± 10 | 90 ± 30 | 430 ± 40 | |
| Duration of Coculture . | Cultured Cells . | No. of Progenitors/Well . | |||
|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Mix . | Total . | ||
| 0 | CD34+CD38+ | 150 ± 25 | 105 ± 15 | 50 ± 5 | 305 ± 40 |
| CD34+CD38− | 25 ± 5 | 10 ± 3 | 30 ± 6 | 65 ± 8 | |
| 4 wk | CD34+CD38+ | 560 ± 40 | 40 ± 5 | 386 ± 45 | 986 ± 90 |
| CD34+CD38− | 120 ± 20 | 20 ± 5 | 136 ± 20 | 276 ± 56 | |
| 6 wk | CD34+CD38+ | 210 ± 40 | 0 | 0 | 210 ± 40 |
| CD34+CD38− | 310 ± 30 | 30 ± 10 | 90 ± 30 | 430 ± 40 | |
AGM-S3 cell layers were established in 24-well plates. Five hundred CD34+CD38+/− cells were planted on stromal cell layers. Clonogenic progenitors generated in the culture at each time point were evaluated by culturing a fraction of cells harvested. The number of colonies indicates the mean ± SD of triplicate cultures.