Coculture of Human CB CD34+ Cells With AGM-S1, 2, and 3 and MS-5 Cells
| Duration of Coculture . | Cell Line . | No. of Progenitors/Well . | |||
|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Mix . | Total . | ||
| 0 | 32 ± 6 | 13 ± 2 | 32 ± 5 | 77 ± 9 | |
| 3 wk | AGM-S 1 | 310 ± 30 | 10 ± 5 | 90 ± 20 | 410 ± 50 |
| AGM-S 2 | 210 ± 25 | 0 | 20 ± 10 | 230 ± 40 | |
| AGM-S 3 | 230 ± 20 | 20 ± 5 | 110 ± 30 | 360 ± 30 | |
| MS-5 | 180 ± 30 | 0 | 0 | 180 ± 30 | |
| 6 wk | AGM-S 1 | 100 ± 12 | 2 ± 2 | 20 ± 8 | 122 ± 16 |
| AGM-S 2 | 24 ± 6 | 0 | 0 | 24 ± 6 | |
| AGM-S 3 | 56 ± 6 | 4 ± 2 | 24 ± 8 | 84 ± 16 | |
| MS-5 | 24 ± 4 | 0 | 0 | 24 ± 4 | |
| Duration of Coculture . | Cell Line . | No. of Progenitors/Well . | |||
|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Mix . | Total . | ||
| 0 | 32 ± 6 | 13 ± 2 | 32 ± 5 | 77 ± 9 | |
| 3 wk | AGM-S 1 | 310 ± 30 | 10 ± 5 | 90 ± 20 | 410 ± 50 |
| AGM-S 2 | 210 ± 25 | 0 | 20 ± 10 | 230 ± 40 | |
| AGM-S 3 | 230 ± 20 | 20 ± 5 | 110 ± 30 | 360 ± 30 | |
| MS-5 | 180 ± 30 | 0 | 0 | 180 ± 30 | |
| 6 wk | AGM-S 1 | 100 ± 12 | 2 ± 2 | 20 ± 8 | 122 ± 16 |
| AGM-S 2 | 24 ± 6 | 0 | 0 | 24 ± 6 | |
| AGM-S 3 | 56 ± 6 | 4 ± 2 | 24 ± 8 | 84 ± 16 | |
| MS-5 | 24 ± 4 | 0 | 0 | 24 ± 4 | |
Cell layers of each line were established in 24-well plates. Five hundred human CB CD34+ cells per well were planted on the stromal cell layers and cultured for 6 weeks. Clonogenic progenitors generated in the culture at each time point were evaluated by culturing a fraction of the cells harvested. The colonies were counted at day 14. The number of colonies indicates the mean ± SD of triplicate cultures.