Coculture of Lin−c-Kit+Sca-1+/− Cells With AGM-S3 Cells
| Cultured Cells . | Days of Coculture . | No. of Progenitors/Well . | ||||
|---|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Mk . | CFU-Mix . | Day-12 CFU-S . | ||
| Lin−c-Kit+Sca-1− | 0 | 15 ± 2 | 26 ± 3 | 0 ± 1 | 0 | 0 |
| 10 | 0 | 0 | 0 | 0 | 0 | |
| Lin−c-Kit+Sca-1+ | 0 | 11 ± 2 | 0 | 4 ± 2 | 24 ± 3 | 3 ± 1 |
| 10 | 2,440 ± 128 | 80 ± 20 | 120 ± 40 | 200 ± 60 | 26 ± 4 | |
| Cultured Cells . | Days of Coculture . | No. of Progenitors/Well . | ||||
|---|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-Mk . | CFU-Mix . | Day-12 CFU-S . | ||
| Lin−c-Kit+Sca-1− | 0 | 15 ± 2 | 26 ± 3 | 0 ± 1 | 0 | 0 |
| 10 | 0 | 0 | 0 | 0 | 0 | |
| Lin−c-Kit+Sca-1+ | 0 | 11 ± 2 | 0 | 4 ± 2 | 24 ± 3 | 3 ± 1 |
| 10 | 2,440 ± 128 | 80 ± 20 | 120 ± 40 | 200 ± 60 | 26 ± 4 | |
Cell layers of AGM-S3 cells were established in 24-well plates. Lin−c-Kit+Sca-1+/− cells (100/well) were planted on AGM-3 cell layers. Clonogenic progenitors generated in the culture at day 10 were evaluated by culturing a fraction of cells harvested. The assay for CFU-S was performed as described in Materials and Methods. The number of colonies indicates the mean ± SD of triplicate cultures.