Coculture of Mouse BMC With AGM-S1, 2, and 3 Cells
| Cell Line . | No. of Cobblestone/Well . | No. of CFU-C/Well . | No. of CFU-S 8/Well . | No. of CFU-S 12/Well . |
|---|---|---|---|---|
| AGM-S 1 | 230 ± 16 | 3,448 ± 131 | 24 ± 4 | 16 ± 2 |
| AGM-S 2 | 126 ± 23 | 768 ± 59 | ND | ND |
| AGM-S 3 | 260 ± 44 | 5,496 ± 184 | 20 ± 2 | 18 ± 3 |
| Cell Line . | No. of Cobblestone/Well . | No. of CFU-C/Well . | No. of CFU-S 8/Well . | No. of CFU-S 12/Well . |
|---|---|---|---|---|
| AGM-S 1 | 230 ± 16 | 3,448 ± 131 | 24 ± 4 | 16 ± 2 |
| AGM-S 2 | 126 ± 23 | 768 ± 59 | ND | ND |
| AGM-S 3 | 260 ± 44 | 5,496 ± 184 | 20 ± 2 | 18 ± 3 |
Cell layers of each line were established in 6-well plates. BMC (1 × 105 per well) were planted on stromal cell layers. BMC (1 × 105) contained 615 ± 40 of CFU-C, 11 ± 2 of day-8 CFU-S, and 6 ± 1 of day-12 CFU-S. The CFU-C were counted at day 8. The number of colonies indicates the mean ± SD of triplicate cultures.
Abbreviation: ND, not determined.