LPS-Induced Differentiation of 2- and 7-Day–Cultured Monocyte-Derived DC
| Marker . | Day of Culture . | Control MFI . | Freshly Isolated % Positive (Δ MFI) . | Medium % Positive (Δ MFI) . | Cells Cultured in: . | ||
|---|---|---|---|---|---|---|---|
| GM-CSF % Positive (Δ MFI) . | GM-CSF/IL-4 % Positive (Δ MFI) . | GM-CSF/IL-4 + LPS % Positive (Δ MFI) . | |||||
| CD14 | 0 | 30.7 | 89.2 (92) | ||||
| 2 | 49.6 | 71.7 (77) | 51.0 (45) | 33.1 (23) | 52.5 (37) | ||
| 7 | 77.6 | 64.6 (46) | 47.9 (21) | 45.4 (17) | 30.8 (9) | ||
| HLA-DR | 0 | 30.7 | 78.8 (55) | ||||
| 2 | 49.6 | 62.0 (50) | 89.8 (75) | 57.1 (48) | 94.1 (70) | ||
| 7 | 77.6 | 41.2 (6) | 31.4 (0) | 80.5 (53) | 86.2 (53) | ||
| HLA-DQ | 0 | 29.4 | 3.9 (7) | ||||
| 2 | 28.9 | 55.9 (43) | 48.3 (39) | 53.2 (46) | 93.4 (78) | ||
| 7 | 78.8 | 84.2 (23) | 84.6 (27) | 89.6 (32) | 96.6 (47) | ||
| CD1a | 0 | 29.4 | 6.7 (10) | ||||
| 2 | 28.9 | 17.0 (2) | 33.6 (29) | 51.4 (36) | 65.1 (53) | ||
| 7 | 78.8 | 79.7 (5) | 77.9 (4) | 97.2 (30) | 95.8 (28) | ||
| CD86 | 0 | 25.7 | 2.0 (16) | ||||
| 2 | 66.5 | 12.3 (0) | 11.0 (0) | 10.3 (0) | 45.3 (33) | ||
| 7 | 71.1 | 41.9 (4) | 55.2 (15) | 46.6 (8) | 94.3 (47) | ||
| Marker . | Day of Culture . | Control MFI . | Freshly Isolated % Positive (Δ MFI) . | Medium % Positive (Δ MFI) . | Cells Cultured in: . | ||
|---|---|---|---|---|---|---|---|
| GM-CSF % Positive (Δ MFI) . | GM-CSF/IL-4 % Positive (Δ MFI) . | GM-CSF/IL-4 + LPS % Positive (Δ MFI) . | |||||
| CD14 | 0 | 30.7 | 89.2 (92) | ||||
| 2 | 49.6 | 71.7 (77) | 51.0 (45) | 33.1 (23) | 52.5 (37) | ||
| 7 | 77.6 | 64.6 (46) | 47.9 (21) | 45.4 (17) | 30.8 (9) | ||
| HLA-DR | 0 | 30.7 | 78.8 (55) | ||||
| 2 | 49.6 | 62.0 (50) | 89.8 (75) | 57.1 (48) | 94.1 (70) | ||
| 7 | 77.6 | 41.2 (6) | 31.4 (0) | 80.5 (53) | 86.2 (53) | ||
| HLA-DQ | 0 | 29.4 | 3.9 (7) | ||||
| 2 | 28.9 | 55.9 (43) | 48.3 (39) | 53.2 (46) | 93.4 (78) | ||
| 7 | 78.8 | 84.2 (23) | 84.6 (27) | 89.6 (32) | 96.6 (47) | ||
| CD1a | 0 | 29.4 | 6.7 (10) | ||||
| 2 | 28.9 | 17.0 (2) | 33.6 (29) | 51.4 (36) | 65.1 (53) | ||
| 7 | 78.8 | 79.7 (5) | 77.9 (4) | 97.2 (30) | 95.8 (28) | ||
| CD86 | 0 | 25.7 | 2.0 (16) | ||||
| 2 | 66.5 | 12.3 (0) | 11.0 (0) | 10.3 (0) | 45.3 (33) | ||
| 7 | 71.1 | 41.9 (4) | 55.2 (15) | 46.6 (8) | 94.3 (47) | ||
Myeloid-enriched non-T cells were either freshly isolated (day 0) or cultured for either 2 or 7 days in either medium, medium supplemented with GM-CSF alone, GM-CSF and IL-4, or GM-CSF and IL-4 with LPS added for the last 24 hours of culture. Cells were stained with various MoAbs or control MoAb, and the % positive and MFI were analyzed. Data are representative of 4 separate experiments.
Abbreviation: Δ MFI, mean fluorescence intensity of sample minus control MFI.