DNA Fragmentation Induced by Cytochrome c in Growing and Quiescent Cells
| . | Pre-G1 . | G1 . | S . | G2/M . | ||||
|---|---|---|---|---|---|---|---|---|
| G . | Q . | G . | Q . | G . | Q . | G . | Q . | |
| Pre-electroporation | ||||||||
| A15 | <1 | 4 | 38 | 36 | 28 | 13 | 33 | 47 |
| B15 | <1 | <1 | 36 | 40 | 28 | 20 | 34 | 36 |
| T = 2 hours | ||||||||
| A15 | ||||||||
| Control | 2 | <1 | 31 | 58 | 29 | 5 | 21 | 28 |
| Cytochrome c | 23 | 5 | 29 | 68 | 28 | 6 | 12(57) | 14 (50) |
| B15 | ||||||||
| Control | 3 | 1 | 42 | 65 | 29 | 12 | 19 | 18 |
| Cytochrome c | 35 | 10 | 30 | 63 | 20 | 12 | 9 (47) | 12 (66) |
| . | Pre-G1 . | G1 . | S . | G2/M . | ||||
|---|---|---|---|---|---|---|---|---|
| G . | Q . | G . | Q . | G . | Q . | G . | Q . | |
| Pre-electroporation | ||||||||
| A15 | <1 | 4 | 38 | 36 | 28 | 13 | 33 | 47 |
| B15 | <1 | <1 | 36 | 40 | 28 | 20 | 34 | 36 |
| T = 2 hours | ||||||||
| A15 | ||||||||
| Control | 2 | <1 | 31 | 58 | 29 | 5 | 21 | 28 |
| Cytochrome c | 23 | 5 | 29 | 68 | 28 | 6 | 12(57) | 14 (50) |
| B15 | ||||||||
| Control | 3 | 1 | 42 | 65 | 29 | 12 | 19 | 18 |
| Cytochrome c | 35 | 10 | 30 | 63 | 20 | 12 | 9 (47) | 12 (66) |
Figures are the percentage of total events; gates are set to include only whole cells (90% to 93% of total events). Figures in parentheses are percent reductions in G2/M with cytochrome c compared with controls. Similar values were obtained at 4 hours. Note that similar reductions in G2/M occurred in growing and quiesced cells with cytochrome c.
Abbreviations: G, growing; Q, quiescent.