Bacterial Uptake and Killing by Normal and PU.1-Null Neutrophils
| . | % Total Viable Bacteria* . | % Viable Intracellular Bacteria† . |
|---|---|---|
| S aureus | ||
| Experiment 1 | ||
| Normal (pooled) | 12.7 ± 2.5 | 2.3 ± 0.6 |
| PU.1 Null #1 | 58.5 ± 1.3 | 34.0 ± 6.6 |
| PU.1 Null #2 | 54.6 ± 1.2 | 46.3 ± 8.1 |
| Experiment 2 | ||
| Normal (pooled) | 27.0 ± 4.6 | 4.0 ± 3.0 |
| PU.1 Null (pooled) | 46.2 ± 0.4 | 48.0 ± 7.1 |
| E. coli | ||
| Experiment 1 | ||
| Normal (pooled) | 15.8 ± 1.9 | 16.2 ± 5.8 |
| PU.1 Null (pooled) | 66.6 ± 7.5 | 33.1 ± 6.1 |
| Experiment 2 | ||
| Normal (pooled) | 18.3 ± 1.4 | 17.3 ± 2.5 |
| PU.1 Null (pooled) | 71.0 ± 1.8 | 54.3 ± 5.3 |
| . | % Total Viable Bacteria* . | % Viable Intracellular Bacteria† . |
|---|---|---|
| S aureus | ||
| Experiment 1 | ||
| Normal (pooled) | 12.7 ± 2.5 | 2.3 ± 0.6 |
| PU.1 Null #1 | 58.5 ± 1.3 | 34.0 ± 6.6 |
| PU.1 Null #2 | 54.6 ± 1.2 | 46.3 ± 8.1 |
| Experiment 2 | ||
| Normal (pooled) | 27.0 ± 4.6 | 4.0 ± 3.0 |
| PU.1 Null (pooled) | 46.2 ± 0.4 | 48.0 ± 7.1 |
| E. coli | ||
| Experiment 1 | ||
| Normal (pooled) | 15.8 ± 1.9 | 16.2 ± 5.8 |
| PU.1 Null (pooled) | 66.6 ± 7.5 | 33.1 ± 6.1 |
| Experiment 2 | ||
| Normal (pooled) | 18.3 ± 1.4 | 17.3 ± 2.5 |
| PU.1 Null (pooled) | 71.0 ± 1.8 | 54.3 ± 5.3 |
*2 ×106 neutrophils were incubated with 107 live S aureus 502A or E coli JM109 (please see Materials and Methods for complete protocols). The percent total viable bacteria were calculated as: (24-hr colony count of intracellular and extracellular bacteria remaining after 2-hr incubation with cells/colony count for Time 0) × 100. Samples were run in triplicate and results are reported as mean ± SD.
After extracellular bacteria were removed by lysis or centrifugation, the percent viable intracellular bacteria remaining was calculated as: (24-hr colony count of intracellular bacteria remaining after 2-hr incubation with cells/colony count for Time 0) × 100. Samples were run in triplicate and results are reported as mean ± SD.