Characterization of MLL-AF4 Fusion Transcripts by Sequence Analysis
| Specimen . | Cytogenetics . | der(11) Fusion Transcript . |
|---|---|---|
| MLLExon/AF4 Exon . | ||
| BM, INF-4 | PD, t(4;11) | MLL 6/AF4 a |
| BM, INF-6 | Normal diploid | MLL 6/AF4 a |
| BM, INF-12 | PD, t(4;11) | MLL 7/AF4 b |
| BM, PEDS-5 | Normal diploid | MLL 7/AF4 b |
| BM, PEDS-27 | PD, t(14;15) | MLL 7/AF4 a |
| BM, PEDS-43 | HID, +SCA | MLL 7/AF4 a |
| BM, PEDS-44 | HID, +SCA | MLL 7/AF4 a |
| BM, PEDS-57 | HID | MLL 7/AF4 b |
| BM, PEDS-58 | HID, del(11)(q23) | MLL 7/AF4 a |
| BM, PEDS-59 | HID, +2-3 mar | MLL 6/AF4 a |
| BM, PEDS-66 | Normal diploid | MLL 6/AF4 a |
| BM, PEDS-69 | Normal diploid | MLL 7/AF4 a |
| BM, PEDS-76 | Normal diploid | MLL 7/AF4 b |
| BM, PEDS-79 | PD | MLL 7/AF4 a |
| BM, PEDS-86 | Normal diploid | MLL 7/AF4 a |
| BM, PEDS-87 | HID, t(4;11) | MLL 6/AF4 a |
| BM, PEDS-91 | PD, t(4;11) | MLL 6/AF4 a |
| BM, PEDS-106 | Normal diploid | MLL 6/AF4 a |
| Fetal liver | 2 (16 wk) | MLL 6/AF4 a |
| Fetal liver | 10 (20 wk) | MLL 7/AF4 b |
| Fetal liver | 12 (21 wk) | MLL 7/AF4 b |
| Fetal liver | 13 (22 wk) | MLL 7/AF4 b |
| Fetal liver | 14 (19 wk) | MLL 6/AF4 a |
| Fetal BM | 4 (19 wk) | MLL 6/AF4 a |
| Fetal BM | 7 (21 wk) | MLL 6/AF4 a |
| Specimen . | Cytogenetics . | der(11) Fusion Transcript . |
|---|---|---|
| MLLExon/AF4 Exon . | ||
| BM, INF-4 | PD, t(4;11) | MLL 6/AF4 a |
| BM, INF-6 | Normal diploid | MLL 6/AF4 a |
| BM, INF-12 | PD, t(4;11) | MLL 7/AF4 b |
| BM, PEDS-5 | Normal diploid | MLL 7/AF4 b |
| BM, PEDS-27 | PD, t(14;15) | MLL 7/AF4 a |
| BM, PEDS-43 | HID, +SCA | MLL 7/AF4 a |
| BM, PEDS-44 | HID, +SCA | MLL 7/AF4 a |
| BM, PEDS-57 | HID | MLL 7/AF4 b |
| BM, PEDS-58 | HID, del(11)(q23) | MLL 7/AF4 a |
| BM, PEDS-59 | HID, +2-3 mar | MLL 6/AF4 a |
| BM, PEDS-66 | Normal diploid | MLL 6/AF4 a |
| BM, PEDS-69 | Normal diploid | MLL 7/AF4 a |
| BM, PEDS-76 | Normal diploid | MLL 7/AF4 b |
| BM, PEDS-79 | PD | MLL 7/AF4 a |
| BM, PEDS-86 | Normal diploid | MLL 7/AF4 a |
| BM, PEDS-87 | HID, t(4;11) | MLL 6/AF4 a |
| BM, PEDS-91 | PD, t(4;11) | MLL 6/AF4 a |
| BM, PEDS-106 | Normal diploid | MLL 6/AF4 a |
| Fetal liver | 2 (16 wk) | MLL 6/AF4 a |
| Fetal liver | 10 (20 wk) | MLL 7/AF4 b |
| Fetal liver | 12 (21 wk) | MLL 7/AF4 b |
| Fetal liver | 13 (22 wk) | MLL 7/AF4 b |
| Fetal liver | 14 (19 wk) | MLL 6/AF4 a |
| Fetal BM | 4 (19 wk) | MLL 6/AF4 a |
| Fetal BM | 7 (21 wk) | MLL 6/AF4 a |
The MLL-AF4 fusion transcripts were further characterized by sequence analysis, as described in detail in Materials and Methods.
Abbreviations: INF, infant; PEDS, pediatrics; PD, pseudodiploid; HID, hyperdiploid; SCA, structure chromosomal abnormalities; NE, not examined.