Percentages of Apoptotic Cells as Calculated From the MiCK Assay and as Measured by Light Microscopy
| Patient No.* . | Leukemia Cell Type . | Drugs (μmol/L) . | KU of Apoptosis . | % of Apoptotic Cells . | |
|---|---|---|---|---|---|
| Calculated . | Observed . | ||||
| 3 | AML | MTA, 0.1 | 0.5 | 7.1 | 8 |
| 6 | MBC/CGL | DNR, 0.5 | 1.23 | 11.4 | 11 |
| 9 | AML/ALL | IDR, 0.5 | 1.25 | 11.5 | 12 |
| 1 | ALL | DNR, 1.0 | 1.3 | 11.8 | 11 |
| 2 | ALL | IDR, 5.0 | 2.31 | 17.9 | 14 |
| 10 | APL | MTA, 2.5 | 3.2 | 23.2 | 21.5 |
| 11 | AML | IDR, 5.0 | 4.25 | 29.5 | 28 |
| 7 | AML/ALL | IDR, 10.0 | 6.5 | 42.9 | 40.5 |
| 12 | AML | MTA, 5.0 | 7.1 | 46.9 | 45.2 |
| 13 | AMMoL | DNR, 20.0 | 8.2 | 53.6 | 51.9 |
| Patient No.* . | Leukemia Cell Type . | Drugs (μmol/L) . | KU of Apoptosis . | % of Apoptotic Cells . | |
|---|---|---|---|---|---|
| Calculated . | Observed . | ||||
| 3 | AML | MTA, 0.1 | 0.5 | 7.1 | 8 |
| 6 | MBC/CGL | DNR, 0.5 | 1.23 | 11.4 | 11 |
| 9 | AML/ALL | IDR, 0.5 | 1.25 | 11.5 | 12 |
| 1 | ALL | DNR, 1.0 | 1.3 | 11.8 | 11 |
| 2 | ALL | IDR, 5.0 | 2.31 | 17.9 | 14 |
| 10 | APL | MTA, 2.5 | 3.2 | 23.2 | 21.5 |
| 11 | AML | IDR, 5.0 | 4.25 | 29.5 | 28 |
| 7 | AML/ALL | IDR, 10.0 | 6.5 | 42.9 | 40.5 |
| 12 | AML | MTA, 5.0 | 7.1 | 46.9 | 45.2 |
| 13 | AMMoL | DNR, 20.0 | 8.2 | 53.6 | 51.9 |
Cells from 7 ANLL, 2 ALL, and 1 AML/ALL patients were exposed to DNR, IDR, or MTA and distributed in two 96-well microtiter plates. One plate was subjected to the MiCK assay while the other was incubated in a CO2-incubator. Apoptosis was measured in KU and determined in cytospin preparations as described in the legend to Fig2. Calculated percentages of apoptotic cells were determined using the equation: Apoptosis (%) = (KU + 0.644)/0.165 [see text].
Patient numbers as they were assigned in Table 1.