Lineage Reconstitution in Lethally Irradiated Mice Transplanted With Uncultured or Cultured Fetal Liver Stem Cells
| Transplanted Cells . | Total % Ly5.1 Cells3-150 . | % Lineage (+) Ly5.1 Cells3-151 . | ||||
|---|---|---|---|---|---|---|
| 4 wk . | 8 wk . | 15 wk . | T Cells . | B Cells . | Myeloid Cells . | |
| Fetal liver cells3-152 (1 × 103cells) | 63.2 59.5 59.6 64.0 | 66.3 70.1 72.8 70.5 | 73.9 77.1 80.0 77.8 | 7.6 5.8 10.4 5.6 | 52.5 56.7 57.1 63.2 | 11.5 12.8 9.5 5.8 |
| Cultured on DAS104-43-153 ( of culture) | 58.1 62.3 51.0 47.2 | 61.3 73.1 63.8 57.2 | 62.9 84.4 69.8 70.6 | 3.9 8.5 5.5 3.3 | 51.3 66.1 54.9 57.1 | 5.8 7.8 7.4 9.0 |
| Cultured on DAS104-8 ( of culture) | 5.3 2.1 2.2 | 1.8 1.7 1.2 | 1.0 0.8 0.7 | ND ND ND | ND ND ND | ND ND ND |
| Cultured on YS CL 72 (of culture) | 13.0 9.7 14.5 | 6.9 25.0 18.8 | 4.7 30.2 11.9 | 0.2 0.8 3.0 | 4.1 25.1 5.9 | 0.1 2.9 2.7 |
| Transplanted Cells . | Total % Ly5.1 Cells3-150 . | % Lineage (+) Ly5.1 Cells3-151 . | ||||
|---|---|---|---|---|---|---|
| 4 wk . | 8 wk . | 15 wk . | T Cells . | B Cells . | Myeloid Cells . | |
| Fetal liver cells3-152 (1 × 103cells) | 63.2 59.5 59.6 64.0 | 66.3 70.1 72.8 70.5 | 73.9 77.1 80.0 77.8 | 7.6 5.8 10.4 5.6 | 52.5 56.7 57.1 63.2 | 11.5 12.8 9.5 5.8 |
| Cultured on DAS104-43-153 ( of culture) | 58.1 62.3 51.0 47.2 | 61.3 73.1 63.8 57.2 | 62.9 84.4 69.8 70.6 | 3.9 8.5 5.5 3.3 | 51.3 66.1 54.9 57.1 | 5.8 7.8 7.4 9.0 |
| Cultured on DAS104-8 ( of culture) | 5.3 2.1 2.2 | 1.8 1.7 1.2 | 1.0 0.8 0.7 | ND ND ND | ND ND ND | ND ND ND |
| Cultured on YS CL 72 (of culture) | 13.0 9.7 14.5 | 6.9 25.0 18.8 | 4.7 30.2 11.9 | 0.2 0.8 3.0 | 4.1 25.1 5.9 | 0.1 2.9 2.7 |
Abbreviation: ND, not determined.
The expression of the donor-type Ly5.1 marker on peripheral blood cells was determined at 4 weeks, 8 weeks, and 15 weeks post transplantation.
The expression of both Ly5.1 donor marker and lineage markers CD4 and CD8 (T cells), B220 (B cells), and Mac-1 and Gr-1 (myeloid cells) was determined on peripheral blood cells at 15 weeks post transplantation.
Day-14 fetal liver cells (Ly5.1) were purified for lin−CD34+Sca-1+c-Kit+stem cells. Purified fetal liver stem cells (1 × 103) were transplanted with 1 × 106 Ly5.2 competitor bone marrow cells into lethally irradiated Ly5.2 mice. Shown are data from individual mice.
3 × 103 fetal liver stem cells were plated onto DAS104-4 and cultured for 7 days. One third of culture (corresponding to 1 × 103 stem cells) was transplanted with 1 × 106 Ly5.2 competitor bone marrow cells into lethally irradiated Ly5.2 mice.