Differentiation of Fetal Liver Stem Cells on AGM-Derived Cell Lines
| Antibody . | % Lineage Positive Cells . | |
|---|---|---|
| DAS104-4 . | DAS104-8 . | |
| Mac-1 | 96.9 ± 0.7 | 93.8 ± 0.9 |
| Gr-1 | 76.0 ± 4.8 | 67.4 ± 4.9 |
| TER-119 | 13.8 ± 2.6 | 14.2 ± 4.0 |
| B220 | 2.6 ± 0.4 | 3.3 ± 4.0 |
| TER-119 (+EPO) | 24.4 ± 0.9 | 71.7 ± 2.7 |
| B220 (+IL-7) | 1.8 ± 0.8 | 92.5 ± 1.6 |
| Antibody . | % Lineage Positive Cells . | |
|---|---|---|
| DAS104-4 . | DAS104-8 . | |
| Mac-1 | 96.9 ± 0.7 | 93.8 ± 0.9 |
| Gr-1 | 76.0 ± 4.8 | 67.4 ± 4.9 |
| TER-119 | 13.8 ± 2.6 | 14.2 ± 4.0 |
| B220 | 2.6 ± 0.4 | 3.3 ± 4.0 |
| TER-119 (+EPO) | 24.4 ± 0.9 | 71.7 ± 2.7 |
| B220 (+IL-7) | 1.8 ± 0.8 | 92.5 ± 1.6 |
Day-14 fetal liver stem cells (lin−CD34+Sca-1+c-Kit+) were plated on each DAS cell line and cultured for 11 to 12 days with or without the indicated added cytokine. Nonadherent cells were obtained by vigorous pipetting and stained with PE-conjugated antibodies as indicated. The frequency of lineage positive cells was determined by FACS in triplicate wells (mean ± SD). TER-119+ cells were analyzed using the red cell gate by FACS.