Transcript Analysis of AGM-Derived Endothelial Cell Lines
| Transcript . | DAS104-4 . | DAS104-8 . |
|---|---|---|
| Endothelial markers | ||
| VEGF | ++ | ++ |
| CD34 | ++ | ++ |
| vWF | ++ | ++ |
| FLK1 | ++ | + |
| FLT1 | ++ | ++ |
| CD31 | ++ | + |
| TIE-2 | +/− | +/− |
| Hematopoietic cytokines | ||
| IL-1 | + | + |
| IL-3 | − | − |
| IL-6 | ++ | ++ |
| IL-11 | ++ | ++ |
| EPO | − | − |
| TPO | + | + |
| LIF | ++ | ++ |
| TGF-β | + | + |
| FLT3 | ++ | ++ |
| SCF | ++ | ++ |
| M-CSF | + | + |
| G-CSF | − | − |
| GM-CSF | ++ | + |
| DLK | ++ | ++ |
| Transcript . | DAS104-4 . | DAS104-8 . |
|---|---|---|
| Endothelial markers | ||
| VEGF | ++ | ++ |
| CD34 | ++ | ++ |
| vWF | ++ | ++ |
| FLK1 | ++ | + |
| FLT1 | ++ | ++ |
| CD31 | ++ | + |
| TIE-2 | +/− | +/− |
| Hematopoietic cytokines | ||
| IL-1 | + | + |
| IL-3 | − | − |
| IL-6 | ++ | ++ |
| IL-11 | ++ | ++ |
| EPO | − | − |
| TPO | + | + |
| LIF | ++ | ++ |
| TGF-β | + | + |
| FLT3 | ++ | ++ |
| SCF | ++ | ++ |
| M-CSF | + | + |
| G-CSF | − | − |
| GM-CSF | ++ | + |
| DLK | ++ | ++ |
Total RNA was isolated from the DAS104-4 and DAS104-8 cell lines and treated with DNAase. Transcripts were measured using specific oligonucleotide primers and 35 cycles of PCR under standard conditions. The resultant reactions were analyzed by agarose gel electrophoresis and ethidium bromide staining.
Abbreviations: ++, strong ethidium bromide stained band; +, weak ethidium bromide stained band; +/−, barely detectable ethidium bromide stained band; −, undetectable ethidium bromide stained band. All positive bands migrated with the appropriate molecular weight.