EGF-Induced Globin Expression in SKT6-EE483, -EE372, and -EENb2 Cells
| EGF Exposure (h) . | SKT6-EE483 . | SKT6-EE372 . | SKT6-EENb2 . | |||
|---|---|---|---|---|---|---|
| W-Blots . | DAF(+) . | W-Blots . | DAF(+) . | W-Blots . | DAF(+) . | |
| 48 | 72% | ND | 154% | ND | 184% | ND |
| 72 | 62% | 66.5% | 152% | 225.5% | 128% | 196.4% |
| EGF Exposure (h) . | SKT6-EE483 . | SKT6-EE372 . | SKT6-EENb2 . | |||
|---|---|---|---|---|---|---|
| W-Blots . | DAF(+) . | W-Blots . | DAF(+) . | W-Blots . | DAF(+) . | |
| 48 | 72% | ND | 154% | ND | 184% | ND |
| 72 | 62% | 66.5% | 152% | 225.5% | 128% | 196.4% |
Values shown are normalized averages versus levels of globin expression induced by Epo in each distinct cell line. Levels of globin expression were assayed quantitatively by high-resolution scanning denstometry of Western blots. Mean values represent percentages of pixel densities for EGF/Epo at each time point (n = 3). Means represent frequencies of DAF (+) cells, and likewise are presented as ratios (EGF-induced/Epo-induced). For Western blots and DAF analyses, observed values ranged 17% and 7% from mean values, respectively.
Abbreviation: ND, not determined.