Competitive Repopulating Ability of Bone Marrow Derived From CD38+/+ and CD38−/− Mice
| Bone Marrow Donor . | % Donor-Derived (Ly 5.2/total) . | |||
|---|---|---|---|---|
| T . | B . | M . | G . | |
| CD38+/+ | 73 ± 13 | 54 ± 9 | 70 ± 11 | 70 ± 11 |
| CD38−/− | 61 ± 6 | 48 ± 8 | 57 ± 9 | 53 ± 12 |
| Bone Marrow Donor . | % Donor-Derived (Ly 5.2/total) . | |||
|---|---|---|---|---|
| T . | B . | M . | G . | |
| CD38+/+ | 73 ± 13 | 54 ± 9 | 70 ± 11 | 70 ± 11 |
| CD38−/− | 61 ± 6 | 48 ± 8 | 57 ± 9 | 53 ± 12 |
Competitive repopulation assays were performed by mixing 5 × 106 bone marrow cells from individual CD38+/+or CD38−/− Ly5.2 mice (129Ola × C57BL/6J), with 5 × 106 competitor bone marrow cells from C57BL/6J Ly5.1 congenic mice. 50:50 mixtures of bone marrow cells were introduced by intravenous injection into irradiated C57BL/6J Ly5.1 congenic recipients, and competitive repopulation was assessed at 12 weeks postreconstitution. The competitive repopulating ability of donor-derived bone marrow was assessed by determining the percent of donor-derived lineage-specific cells among all cells within that lineage. Cell numbers within the T-cell (T), B-cell (B), macrophage (M), and granulocyte (G) lineages were determined by FACS analysis of peripheral blood from individual recipient mice as described in the Materials and Methods. Data shown represent the mean ± standard deviation of the percent donor reconstitution of three individual mice.