Repopulating Abilities of Freshly Prepared Bone Marrow Cells and Cultured Cells
| Additional Cytokines . | Cells . | Fractions of Cells Injected . | % Donor PB Cells4-150 . | |
|---|---|---|---|---|
| 2 mo . | 6 mo . | |||
| No culture | 100 | 1 | 51.4 ± 17.2 | 65.2 ± 26.3 |
| 1/10 | 16.7 ± 26.7 | 25.7 ± 30.3 | ||
| None | 1.4 × 105 | 1 | 65.0 ± 3.6 | 48.5 ± 2.2 |
| 1/10 | 19.0 ± 8.0 | 4.9 ± 3.2 | ||
| GM-CSF | 3.5 × 105 | 1 | 64.3 ± 12.1 | 50.4 ± 29.0 |
| 1/10 | 8.8 ± 6.0 | 5.5 ± 4.4 | ||
| Additional Cytokines . | Cells . | Fractions of Cells Injected . | % Donor PB Cells4-150 . | |
|---|---|---|---|---|
| 2 mo . | 6 mo . | |||
| No culture | 100 | 1 | 51.4 ± 17.2 | 65.2 ± 26.3 |
| 1/10 | 16.7 ± 26.7 | 25.7 ± 30.3 | ||
| None | 1.4 × 105 | 1 | 65.0 ± 3.6 | 48.5 ± 2.2 |
| 1/10 | 19.0 ± 8.0 | 4.9 ± 3.2 | ||
| GM-CSF | 3.5 × 105 | 1 | 64.3 ± 12.1 | 50.4 ± 29.0 |
| 1/10 | 8.8 ± 6.0 | 5.5 ± 4.4 | ||
Irradiated recipients (five mice per group) were transplanted with 100 or 10 freshly prepared cells or with fractions of day-7 cultures that had been initiated with 100 enriched cells. These cells had been expanded in culture in the presence of SF, IL-6, IL-11, EPO, and the designated additional cytokines. Background (peripheral blood of nontransplanted female mice) staining was less than 1.0%.
Abbreviation: PB, peripheral blood.
Mean ± SD of percent donor-derived nucleated peripheral blood cells tested with anti–Ly-5.1 MoAb (A20-1.7).