Effects of GM-CSF on Lymphohematopoietic Progenitors
| Cytokines . | Replated on Day 8 . | Replated on Day 11 . | |
|---|---|---|---|
| No. of 1° Colonies . | No. of Pre–B-Cell Colonies . | No. of Pre–B-Cell Colonies . | |
| SF, IL-11 | 27 ± 2 | 44 ± 2 | 29 ± 3 |
| SF, IL-11, IL-3 (1 ng/mL) | 26 ± 3 | 0 | 0 |
| SF, IL-11, IL-3 (10 ng/mL) | 26 ± 4 | 0 | 0 |
| SF, IL-11, IL-3 (100 ng/mL) | 25 ± 3 | 0 | 0 |
| SF, IL-11, GM-CSF (10 ng/mL) | 27 ± 1 | 45 ± 2 | 31 ± 5 |
| SF, IL-11, GM-CSF (100 ng/mL) | 28 ± 4 | 44 ± 5 | 32 ± 8 |
| SF, IL-11, GM-CSF (1,000 ng/mL) | 27 ± 4 | 45 ± 2 | 29 ± 4 |
| SF, IL-6 | 25 ± 3 | 33 ± 4 | 51 ± 3 |
| SF, IL-6, IL-3 (1 ng/mL) | 28 ± 3 | 0 | 0 |
| SF, IL-6, IL-3 (10 ng/mL) | 27 ± 5 | 0 | 0 |
| SF, IL-6, IL-3 (100 ng/mL) | 28 ± 3 | 0 | 0 |
| SF, IL-6, GM-CSF (10 ng/mL) | 27 ± 3 | 31 ± 2 | 53 ± 5 |
| SF, IL-6, GM-CSF (100 ng/mL) | 28 ± 6 | 30 ± 3 | 51 ± 7 |
| SF, IL-6, GM-CSF (1,000 ng/mL) | 24 ± 4 | 31 ± 4 | 49 ± 7 |
| SF, G-CSF | 23 ± 6 | 55 ± 6 | 21 ± 3 |
| SF, G-CSF, IL-3 (1 ng/mL) | 25 ± 2 | 0 | 0 |
| SF, G-CSF, IL-3 (10 ng/mL) | 26 ± 2 | 0 | 0 |
| SF, G-CSF, IL-3 (100 ng/mL) | 25 ± 4 | 0 | 0 |
| SF, G-CSF, GM-CSF (10 ng/mL) | 23 ± 7 | 53 ± 9 | 20 ± 3 |
| SF, G-CSF, GM-CSF (100 ng/mL) | 25 ± 2 | 46 ± 6 | 21 ± 2 |
| SF, G-CSF, GM-CSF (1,000 ng/mL) | 25 ± 5 | 53 ± 5 | 21 ± 3 |
| Cytokines . | Replated on Day 8 . | Replated on Day 11 . | |
|---|---|---|---|
| No. of 1° Colonies . | No. of Pre–B-Cell Colonies . | No. of Pre–B-Cell Colonies . | |
| SF, IL-11 | 27 ± 2 | 44 ± 2 | 29 ± 3 |
| SF, IL-11, IL-3 (1 ng/mL) | 26 ± 3 | 0 | 0 |
| SF, IL-11, IL-3 (10 ng/mL) | 26 ± 4 | 0 | 0 |
| SF, IL-11, IL-3 (100 ng/mL) | 25 ± 3 | 0 | 0 |
| SF, IL-11, GM-CSF (10 ng/mL) | 27 ± 1 | 45 ± 2 | 31 ± 5 |
| SF, IL-11, GM-CSF (100 ng/mL) | 28 ± 4 | 44 ± 5 | 32 ± 8 |
| SF, IL-11, GM-CSF (1,000 ng/mL) | 27 ± 4 | 45 ± 2 | 29 ± 4 |
| SF, IL-6 | 25 ± 3 | 33 ± 4 | 51 ± 3 |
| SF, IL-6, IL-3 (1 ng/mL) | 28 ± 3 | 0 | 0 |
| SF, IL-6, IL-3 (10 ng/mL) | 27 ± 5 | 0 | 0 |
| SF, IL-6, IL-3 (100 ng/mL) | 28 ± 3 | 0 | 0 |
| SF, IL-6, GM-CSF (10 ng/mL) | 27 ± 3 | 31 ± 2 | 53 ± 5 |
| SF, IL-6, GM-CSF (100 ng/mL) | 28 ± 6 | 30 ± 3 | 51 ± 7 |
| SF, IL-6, GM-CSF (1,000 ng/mL) | 24 ± 4 | 31 ± 4 | 49 ± 7 |
| SF, G-CSF | 23 ± 6 | 55 ± 6 | 21 ± 3 |
| SF, G-CSF, IL-3 (1 ng/mL) | 25 ± 2 | 0 | 0 |
| SF, G-CSF, IL-3 (10 ng/mL) | 26 ± 2 | 0 | 0 |
| SF, G-CSF, IL-3 (100 ng/mL) | 25 ± 4 | 0 | 0 |
| SF, G-CSF, GM-CSF (10 ng/mL) | 23 ± 7 | 53 ± 9 | 20 ± 3 |
| SF, G-CSF, GM-CSF (100 ng/mL) | 25 ± 2 | 46 ± 6 | 21 ± 2 |
| SF, G-CSF, GM-CSF (1,000 ng/mL) | 25 ± 5 | 53 ± 5 | 21 ± 3 |
One hundred Lin− Sca-1+c-kit+ cells from the marrow of 5-FU–treated wild-type mice were cultured in methylcellulose with designated cytokines. Eight and 11 days later, 20 primary colonies were pooled and 5% of the cells were replated in methylcellulose with SF and IL-7. The pre–B-cell colony formation was examined on day 10 of secondary culture. Similar results were obtained in an additional experiment.