Lineage Expression by Engrafted Donor (Ly-5.2) Cells in Individual Mice at 6 Months Posttransplantation
| Mice . | Cytokines . | Mice . | % B220+ Cells . | % Thy-1.2+ Cells . | % Gr-1+ Mac-1+ Cells . |
|---|---|---|---|---|---|
| Wild-type | No culture | 1 | 35.4 | 16.9 | 22.9 |
| (βc +/+, βIL-3 +/+) | 2 | 43.8 | 12.1 | 20.9 | |
| 3 | 19.3 | 10.8 | 11.6 | ||
| 4 | 29.2 | 13.2 | 20.5 | ||
| 5 | 16.6 | 14.3 | 15.2 | ||
| 6 | 36.2 | 18.0 | 16.8 | ||
| 7 | 33.9 | 22.0 | 24.6 | ||
| SF, IL-11 | 1 | 11.4 | 4.8 | 2.4 | |
| 2 | 21.3 | 15.5 | 12.4 | ||
| 3 | 38.0 | 22.2 | 14.5 | ||
| 4 | 22.6 | 12.5 | 4.9 | ||
| 5 | 39.4 | 23.7 | 23.6 | ||
| 6 | 34.1 | 19.7 | 19.3 | ||
| 7 | 22.4 | 16.6 | 14.6 | ||
| βIL-3 −/− | No culture | 1 | 27.6 | 17.7 | 15.4 |
| 2 | 34.2 | 18.1 | 15.9 | ||
| 3 | 39.1 | 20.8 | 18.9 | ||
| 4 | 11.9 | 6.5 | 8.7 | ||
| 5 | 15.7 | 8.1 | 3.1 | ||
| 6 | 14.8 | 10.9 | 3.9 | ||
| 7 | 30.9 | 26.1 | 18.1 | ||
| SF, IL-11 | 1 | 20.7 | 16.1 | 10.0 | |
| 2 | 8.6 | 3.9 | 2.7 | ||
| 3 | 24.6 | 17.4 | 10.5 | ||
| 4 | 13.8 | 13.5 | 11.3 | ||
| 5 | 24.3 | 22.3 | 7.4 | ||
| 6 | 26.1 | 19.6 | 16.7 | ||
| 7 | 14.9 | 4.3 | 3.2 | ||
| βc −/− | No culture | 1 | 10.6 | 5.1 | 2.9 |
| 2 | 46.1 | 22.9 | 15.8 | ||
| 3 | 36.3 | 12.0 | 6.6 | ||
| 4 | 26.0 | 11.2 | 6.6 | ||
| 5 | 17.3 | 6.4 | 4.2 | ||
| 6 | 23.5 | 13.7 | 23.2 | ||
| 7 | 25.7 | 13.0 | 11.9 | ||
| SF, IL-11 | 1 | 30.0 | 23.7 | 11.6 | |
| 2 | 24.7 | 11.7 | 4.9 | ||
| 3 | 19.5 | 9.7 | 6.8 | ||
| 4 | 25.5 | 10.0 | 9.4 | ||
| 5 | 18.5 | 16.4 | 12.0 | ||
| 6 | 10.7 | 7.6 | 5.8 | ||
| 7 | 16.7 | 11.6 | 2.9 |
| Mice . | Cytokines . | Mice . | % B220+ Cells . | % Thy-1.2+ Cells . | % Gr-1+ Mac-1+ Cells . |
|---|---|---|---|---|---|
| Wild-type | No culture | 1 | 35.4 | 16.9 | 22.9 |
| (βc +/+, βIL-3 +/+) | 2 | 43.8 | 12.1 | 20.9 | |
| 3 | 19.3 | 10.8 | 11.6 | ||
| 4 | 29.2 | 13.2 | 20.5 | ||
| 5 | 16.6 | 14.3 | 15.2 | ||
| 6 | 36.2 | 18.0 | 16.8 | ||
| 7 | 33.9 | 22.0 | 24.6 | ||
| SF, IL-11 | 1 | 11.4 | 4.8 | 2.4 | |
| 2 | 21.3 | 15.5 | 12.4 | ||
| 3 | 38.0 | 22.2 | 14.5 | ||
| 4 | 22.6 | 12.5 | 4.9 | ||
| 5 | 39.4 | 23.7 | 23.6 | ||
| 6 | 34.1 | 19.7 | 19.3 | ||
| 7 | 22.4 | 16.6 | 14.6 | ||
| βIL-3 −/− | No culture | 1 | 27.6 | 17.7 | 15.4 |
| 2 | 34.2 | 18.1 | 15.9 | ||
| 3 | 39.1 | 20.8 | 18.9 | ||
| 4 | 11.9 | 6.5 | 8.7 | ||
| 5 | 15.7 | 8.1 | 3.1 | ||
| 6 | 14.8 | 10.9 | 3.9 | ||
| 7 | 30.9 | 26.1 | 18.1 | ||
| SF, IL-11 | 1 | 20.7 | 16.1 | 10.0 | |
| 2 | 8.6 | 3.9 | 2.7 | ||
| 3 | 24.6 | 17.4 | 10.5 | ||
| 4 | 13.8 | 13.5 | 11.3 | ||
| 5 | 24.3 | 22.3 | 7.4 | ||
| 6 | 26.1 | 19.6 | 16.7 | ||
| 7 | 14.9 | 4.3 | 3.2 | ||
| βc −/− | No culture | 1 | 10.6 | 5.1 | 2.9 |
| 2 | 46.1 | 22.9 | 15.8 | ||
| 3 | 36.3 | 12.0 | 6.6 | ||
| 4 | 26.0 | 11.2 | 6.6 | ||
| 5 | 17.3 | 6.4 | 4.2 | ||
| 6 | 23.5 | 13.7 | 23.2 | ||
| 7 | 25.7 | 13.0 | 11.9 | ||
| SF, IL-11 | 1 | 30.0 | 23.7 | 11.6 | |
| 2 | 24.7 | 11.7 | 4.9 | ||
| 3 | 19.5 | 9.7 | 6.8 | ||
| 4 | 25.5 | 10.0 | 9.4 | ||
| 5 | 18.5 | 16.4 | 12.0 | ||
| 6 | 10.7 | 7.6 | 5.8 | ||
| 7 | 16.7 | 11.6 | 2.9 |
Irradiated recipients (seven mice per group) were transplanted with freshly sorted 200 Lin− Sca-1+c-kit+ cells or with all of the cultures expanded from 200 Lin− Sca-1+ c-kit+ cells. Background (peripheral blood of nontransplanted male mice) staining by anti–Ly-5.2 MoAb (AL1-4A2) was less than 1.0%.