Effects of Cytokines on the Proliferation of HB-1 Cells
| Interleukin . | Day 6 (×104 no. of cells) . | Ratio . |
|---|---|---|
| HB-1 on MS-10 | 63.0 ± 1.0 | 100 |
| HB-1 in α-MEM alone | 1.4 ± 0.2 | 2.2 |
| rhIL-1α (10 U/mL) | 56.0 ± 2.0 | 88.9 |
| rhIL-1α (10 U/mL) + IL-3 (15% STIL-3C5)* (120 U) | 57.0 ± 4.0 | 95.0 |
| rhIL-1α (10 U/mL) + rmIL-6 (10 U/mL) | 52.5 ± 1.3 | 83.3 |
| rhIL-1α (10 U/mL) + rhIL-11 (20 U/mL) | 101.0 ± 5.2 | 160.0 |
| rhIL-1α (10 U/mL) + rmGM-CSF (250 U/mL) | 58.2 ± 3.4 | 92.9 |
| IL-3 (15% STIL-3C5)* (120 U/mL) | 1.4 ± 0.3 | 2.3 |
| rmIL-6 (10 U/mL) | 1.7 ± 0.5 | 2.8 |
| rhIL-11 (20 U/mL) | 1.8 ± 0.4 | 2.9 |
| rmGM-CSF (250 U/mL) | 1.6 ± 0.3 | 2.5 |
| Interleukin . | Day 6 (×104 no. of cells) . | Ratio . |
|---|---|---|
| HB-1 on MS-10 | 63.0 ± 1.0 | 100 |
| HB-1 in α-MEM alone | 1.4 ± 0.2 | 2.2 |
| rhIL-1α (10 U/mL) | 56.0 ± 2.0 | 88.9 |
| rhIL-1α (10 U/mL) + IL-3 (15% STIL-3C5)* (120 U) | 57.0 ± 4.0 | 95.0 |
| rhIL-1α (10 U/mL) + rmIL-6 (10 U/mL) | 52.5 ± 1.3 | 83.3 |
| rhIL-1α (10 U/mL) + rhIL-11 (20 U/mL) | 101.0 ± 5.2 | 160.0 |
| rhIL-1α (10 U/mL) + rmGM-CSF (250 U/mL) | 58.2 ± 3.4 | 92.9 |
| IL-3 (15% STIL-3C5)* (120 U/mL) | 1.4 ± 0.3 | 2.3 |
| rmIL-6 (10 U/mL) | 1.7 ± 0.5 | 2.8 |
| rhIL-11 (20 U/mL) | 1.8 ± 0.4 | 2.9 |
| rmGM-CSF (250 U/mL) | 1.6 ± 0.3 | 2.5 |
A total of 4 × 104 HB-1 cells were cultured in the presence of each cytokine or on MS-10 cells, and cell numbers were counted at day 6. Ratio represents cell number of each sample to number of HB-1 cells on MS-10 cells on day 6.
Fifteen percent of STIL-3 CM was used. The STIL-3 C5 CM contained about 800 U/mL of IL-3 activity as determined by mast cell-colony formation assay.15 All results represent mean ± SE.