Hematological Analysis of the Mice Heterozygous to the GATA-1.05 Allele
| No. . | Hematocrit (%) . | Platelet (1 × 104) . | No. of Megakaryocytes (bone marrow) . | No. of Megakaryocytes (spleen) . |
|---|---|---|---|---|
| Wild-type mouse (8-12 weeks old) | ||||
| 1 | 42 | 144 | 151 | 19 |
| 2 | 36 | 155 | 158 | 5 |
| 3 | 42 | 145 | 183 | 6 |
| 4 | 41 | 91.5 | 25 | 0 |
| 5 | 46 | 116 | 50 | 1 |
| 6 | 50 | 122 | 52 | 5 |
| Mean | 42.8-150 | 128.9-150,-151 | 103.2 | 6.0-151 |
| GATA-1.05 heterozygous mouse (8-12 weeks old) | ||||
| 1 | 37 | 100 | 258 | 100 |
| 2 | 40 | 79 | 144 | 49 |
| 3 | 42 | 55 | 405 | 47 |
| 4 | 52 | 72 | 32 | 9 |
| 5 | 47 | 99.5 | 12 | 5 |
| 6 | 52 | 76.5 | 71 | 5 |
| 7 | ND | ND | 70 | 6 |
| 8 | 38 | 69.5 | 59 | 3 |
| 9 | 44 | 66.6 | 155 | 2 |
| 10 | 43 | 69.1 | 113 | 4 |
| 11 | ND | ND | 184 | 16 |
| Mean | 43.9 | 76.3-151 | 136.6 | 22.3 |
| GATA-1.05 heterozygous mouse (20 weeks old) | ||||
| 12-152 | 25 | 46 | 165 | 18 |
| 13-152 | 30 | 50 | 180 | 30 |
| 14-152 | 27 | 63 | ND | ND |
| Mean | 27.3-150 | 53.0-150 | 172.5 | 24.0-151 |
| No. . | Hematocrit (%) . | Platelet (1 × 104) . | No. of Megakaryocytes (bone marrow) . | No. of Megakaryocytes (spleen) . |
|---|---|---|---|---|
| Wild-type mouse (8-12 weeks old) | ||||
| 1 | 42 | 144 | 151 | 19 |
| 2 | 36 | 155 | 158 | 5 |
| 3 | 42 | 145 | 183 | 6 |
| 4 | 41 | 91.5 | 25 | 0 |
| 5 | 46 | 116 | 50 | 1 |
| 6 | 50 | 122 | 52 | 5 |
| Mean | 42.8-150 | 128.9-150,-151 | 103.2 | 6.0-151 |
| GATA-1.05 heterozygous mouse (8-12 weeks old) | ||||
| 1 | 37 | 100 | 258 | 100 |
| 2 | 40 | 79 | 144 | 49 |
| 3 | 42 | 55 | 405 | 47 |
| 4 | 52 | 72 | 32 | 9 |
| 5 | 47 | 99.5 | 12 | 5 |
| 6 | 52 | 76.5 | 71 | 5 |
| 7 | ND | ND | 70 | 6 |
| 8 | 38 | 69.5 | 59 | 3 |
| 9 | 44 | 66.6 | 155 | 2 |
| 10 | 43 | 69.1 | 113 | 4 |
| 11 | ND | ND | 184 | 16 |
| Mean | 43.9 | 76.3-151 | 136.6 | 22.3 |
| GATA-1.05 heterozygous mouse (20 weeks old) | ||||
| 12-152 | 25 | 46 | 165 | 18 |
| 13-152 | 30 | 50 | 180 | 30 |
| 14-152 | 27 | 63 | ND | ND |
| Mean | 27.3-150 | 53.0-150 | 172.5 | 24.0-151 |
Hematocrit value and platelet number were examined routinely for the GATA-1.05 heterozygous mice. Numbers of megakaryocytes were determined by biopsy. Biopsy of overtly healthy GATA-1.05heterozygous mutant females was performed on a group of 11 young mice of 8 to 12 weeks of age. Six wild-type littermates were used as the control.
Abbreviation: ND, not determined.
P < .001.
P < .05.
Mice analyzed at 20 weeks after birth that showed marked anemia upon routine hematological examination. Numbers of megakaryocytes in these mice were determined upon necropsy.