Sequence and Position of Primers Used to Amplify cDNA and Genomic DNA
| PCR Products . | Primer . | Nucleotide Sequence . | Position (nt)-150 . |
|---|---|---|---|
| cDNA | |||
| nt −19 to 1,350 | J (s) | 5′-GCACAGAGACGGACACAG-3′ | −19 to −2 |
| K (a) | 5′-TGCTGTCATGAGCGTTTCTCA-3′ | 1,350 to 1,330 | |
| nt 337 to 939 | L (s) | 5′-ATTCGGCTGGCCACCATGAGT-3′ | 337 to 357 |
| M (a) | 5′-CGGCAGGCACTTGGCTCC-3′ | 939 to 922 | |
| Genomic DNA | |||
| Exon 1-intron 1 | N (s) | 5′-GTCCGGCGCTGCCTGCCC-3′ | 25 to 42 |
| I1 (a) | 5′-TGCTCCTGTGACCACTGT-3′ | 34 nt down-151 ex 1 | |
| Intron 1-intron 2 | I2 (s) | 5′-CCCTGCCATTTAGTAAGACTC-3′ | 89 nt up-151 ex 2 |
| I3 (a) | 5′-TACAGGATGCCCAGTTAATTTG-3′ | 183 nt down-151 ex 2 | |
| Intron 2-intron 3 | I4 (s) | 5′-AAAGTAAGGTGCCAACAGT-3′ | 104 nt up-151ex3 |
| I5 (a) | 5′-CCCCACCAAATGGAGCTT-3′ | 62 nt down-151 ex 3 | |
| Intron 3-exon 4 | I6 (s) | 5′-GCTTCTGAGCAGAGGAG-3′ | 1,523 nt up-151 ex 4 |
| O (a) | 5′-GAACACGTAGAAGTGCCTCAG-3′ | 525 to 505 | |
| Exon 4-exon 6 | P (s) | 5′-ACTACCACATGAACCTGAGGCACT-3′ | 491 to 514 |
| M (a) | 5′-CGGCAGGCACTTGGCTCC-3′ | 939 to 922 | |
| Exon 6-exon 7 | R (s) | 5′-GTGGGTACCTCGTGTCACCTGATC-3′ | 841 to 864 |
| S (a) | 5′-GCCATTGCCGTTCCAGACAGTATG-3′ | 1,071 to 1,048 |
| PCR Products . | Primer . | Nucleotide Sequence . | Position (nt)-150 . |
|---|---|---|---|
| cDNA | |||
| nt −19 to 1,350 | J (s) | 5′-GCACAGAGACGGACACAG-3′ | −19 to −2 |
| K (a) | 5′-TGCTGTCATGAGCGTTTCTCA-3′ | 1,350 to 1,330 | |
| nt 337 to 939 | L (s) | 5′-ATTCGGCTGGCCACCATGAGT-3′ | 337 to 357 |
| M (a) | 5′-CGGCAGGCACTTGGCTCC-3′ | 939 to 922 | |
| Genomic DNA | |||
| Exon 1-intron 1 | N (s) | 5′-GTCCGGCGCTGCCTGCCC-3′ | 25 to 42 |
| I1 (a) | 5′-TGCTCCTGTGACCACTGT-3′ | 34 nt down-151 ex 1 | |
| Intron 1-intron 2 | I2 (s) | 5′-CCCTGCCATTTAGTAAGACTC-3′ | 89 nt up-151 ex 2 |
| I3 (a) | 5′-TACAGGATGCCCAGTTAATTTG-3′ | 183 nt down-151 ex 2 | |
| Intron 2-intron 3 | I4 (s) | 5′-AAAGTAAGGTGCCAACAGT-3′ | 104 nt up-151ex3 |
| I5 (a) | 5′-CCCCACCAAATGGAGCTT-3′ | 62 nt down-151 ex 3 | |
| Intron 3-exon 4 | I6 (s) | 5′-GCTTCTGAGCAGAGGAG-3′ | 1,523 nt up-151 ex 4 |
| O (a) | 5′-GAACACGTAGAAGTGCCTCAG-3′ | 525 to 505 | |
| Exon 4-exon 6 | P (s) | 5′-ACTACCACATGAACCTGAGGCACT-3′ | 491 to 514 |
| M (a) | 5′-CGGCAGGCACTTGGCTCC-3′ | 939 to 922 | |
| Exon 6-exon 7 | R (s) | 5′-GTGGGTACCTCGTGTCACCTGATC-3′ | 841 to 864 |
| S (a) | 5′-GCCATTGCCGTTCCAGACAGTATG-3′ | 1,071 to 1,048 |