Primers and Probes Used for TaqMan Real-Time Quantitative RT-PCR
| TaqMan probe: TPO cDNA-518T | 5′-GAGAATGGAAAACCCAGATGGA-3′ |
| GAPDH amolicon | 5′-GAAGGTGAAGGTCGGAGT-3′ |
| PCR primer: TPO cDNA-310F (forward) | 5′-GTGGACCCTCCTACAAGCATCA-3′ |
| TPO cDNA-610R (reverse) | 5′-GTGGACCCTCCTACAAGCATCA-3′ |
| GAPDH cDNA (forward) | 5′-GAAGGTGAAGGTCGGAGT-3′ |
| GAPDH cDNA (reverse) | 5′-GAAGATGGTGATGGGA-3′ |
| TaqMan probe: TPO cDNA-518T | 5′-GAGAATGGAAAACCCAGATGGA-3′ |
| GAPDH amolicon | 5′-GAAGGTGAAGGTCGGAGT-3′ |
| PCR primer: TPO cDNA-310F (forward) | 5′-GTGGACCCTCCTACAAGCATCA-3′ |
| TPO cDNA-610R (reverse) | 5′-GTGGACCCTCCTACAAGCATCA-3′ |
| GAPDH cDNA (forward) | 5′-GAAGGTGAAGGTCGGAGT-3′ |
| GAPDH cDNA (reverse) | 5′-GAAGATGGTGATGGGA-3′ |