Differential Counts of BM Cells From Untreated and G-CSF–Treated Mice
| Cell Type . | Nonstimulated . | G-CSF–Stimulated . | ||||
|---|---|---|---|---|---|---|
| wt/wt . | wt/Δ715 . | Δ715/Δ715 . | wt/wt . | wt/Δ715 . | Δ715/Δ715 . | |
| Total nucleated (×106) | 99 ± 20 | 101 ± 25 | 111 ± 31 | 94 ± 7 | 103 ± 10 | 112 ± 5 |
| Percentage | ||||||
| Myeloblast | 0.4 ± 0.5 | 0.5 ± 0.6 | 0.5 ± 0.3 | 0.8 ± 0.6 | 0.2 ± 0.2 | 1.1 ± 0.5 |
| Promyelocyte | 5.5 ± 0.8 | 4.9 ± 1.3 | 5.0 ± 1.6 | 7.7 ± 1.6 | 8.6 ± 2.1 | 13.2 ± 3.3 |
| Myelocyte | 6.5 ± 2.2 | 7.3 ± 3.1 | 7.7 ± 1.9 | 6.3 ± 3.3 | 4.1 ± 1.6 | 11.7 ± 2.3 |
| Metamyelocyte | 4.2 ± 1.4 | 3.9 ± 1.5 | 4.8 ± 1.0 | 3.5 ± 1.8 | 3.9 ± 0.4 | 6.4 ± 0.5 |
| Band form | 0.8 ± 0.2 | 1.0 ± 0.8 | 1.0 ± 0.5 | 3.1 ± 0.6 | 2.4 ± 0.6 | 4.2 ± 1.0 |
| Segment | 32.5 ± 8.0 | 30.9 ± 9.3 | 29.1 ± 5.9 | 63.6 ± 9.4 | 74.3 ± 4.7 | 52.6 ± 8.7 |
| Eosinophil | 2.0 ± 1.3 | 3.3 ± 1.2 | 2.2 ± 1.0 | 3.0 ± 2.1 | 0.3 ± 0.6 | 4.5 ± 1.7 |
| Monocyte | 1.2 ± 0.1 | 0.8 ± 0.4 | 1.3 ± 0.5 | 0.6 ± 0.1 | 0 ± 0 | 0.3 ± 0.2 |
| Erythroid | 23.7 ± 6.9 | 25.0 ± 1.5 | 26.2 ± 3.5 | 7.2 ± 3.5 | 2.2 ± 0.3 | 3.5 ± 2.9 |
| Lymphoid | 23.2 ± 2.2 | 22.3 ± 2.1 | 21.8 ± 2.1 | 6.4 ± 3.4 | 3.8 ± 1.3 | 6.5 ± 1.8 |
| Cell Type . | Nonstimulated . | G-CSF–Stimulated . | ||||
|---|---|---|---|---|---|---|
| wt/wt . | wt/Δ715 . | Δ715/Δ715 . | wt/wt . | wt/Δ715 . | Δ715/Δ715 . | |
| Total nucleated (×106) | 99 ± 20 | 101 ± 25 | 111 ± 31 | 94 ± 7 | 103 ± 10 | 112 ± 5 |
| Percentage | ||||||
| Myeloblast | 0.4 ± 0.5 | 0.5 ± 0.6 | 0.5 ± 0.3 | 0.8 ± 0.6 | 0.2 ± 0.2 | 1.1 ± 0.5 |
| Promyelocyte | 5.5 ± 0.8 | 4.9 ± 1.3 | 5.0 ± 1.6 | 7.7 ± 1.6 | 8.6 ± 2.1 | 13.2 ± 3.3 |
| Myelocyte | 6.5 ± 2.2 | 7.3 ± 3.1 | 7.7 ± 1.9 | 6.3 ± 3.3 | 4.1 ± 1.6 | 11.7 ± 2.3 |
| Metamyelocyte | 4.2 ± 1.4 | 3.9 ± 1.5 | 4.8 ± 1.0 | 3.5 ± 1.8 | 3.9 ± 0.4 | 6.4 ± 0.5 |
| Band form | 0.8 ± 0.2 | 1.0 ± 0.8 | 1.0 ± 0.5 | 3.1 ± 0.6 | 2.4 ± 0.6 | 4.2 ± 1.0 |
| Segment | 32.5 ± 8.0 | 30.9 ± 9.3 | 29.1 ± 5.9 | 63.6 ± 9.4 | 74.3 ± 4.7 | 52.6 ± 8.7 |
| Eosinophil | 2.0 ± 1.3 | 3.3 ± 1.2 | 2.2 ± 1.0 | 3.0 ± 2.1 | 0.3 ± 0.6 | 4.5 ± 1.7 |
| Monocyte | 1.2 ± 0.1 | 0.8 ± 0.4 | 1.3 ± 0.5 | 0.6 ± 0.1 | 0 ± 0 | 0.3 ± 0.2 |
| Erythroid | 23.7 ± 6.9 | 25.0 ± 1.5 | 26.2 ± 3.5 | 7.2 ± 3.5 | 2.2 ± 0.3 | 3.5 ± 2.9 |
| Lymphoid | 23.2 ± 2.2 | 22.3 ± 2.1 | 21.8 ± 2.1 | 6.4 ± 3.4 | 3.8 ± 1.3 | 6.5 ± 1.8 |
Cells were recovered from both femurs and tibias of 10-week-old mice and counted. At least 500 cells were differentially scored for their morphology. Data are means of 4 mice ± SD.