Percentage of CD8α+ or CD8αβ+ Cells Within the CD8α+ T Cell, CD8αbright T Cell, NK Cell, TCRγδ+ T Cell, and CD4+ T-Cell Subsets
| Gated Cells . | Assessed for Expression of: . | HIV-1 Seronegative (n = 21) . | HIV-1 Seropositive >200 CD4+cells/μL (n = 58) . | HIV-1 Seropositive <200 CD4+ cells/μL (n = 45) . |
|---|---|---|---|---|
| Median % of CD8α+ or CD8αβ+ cell subset within gated cells (25%, 75%) . | ||||
| CD8α+ T cells | CD8αβ | 90.8 (81.1, 92.6) | 96.9 (94.8, 98.0)* | 97.6 (95.3, 98.3)* |
| CD8αbright T cells | CD8αβ | 97.1 (94.7, 98.3) | 99.4 (98.6, 99.6)* | 99.2 (98.6, 99.6)* |
| NK cells | CD8α | 46.7 (41.1, 60.7) | 28.2 (23.2, 35.3)* | 25.2 (19.2, 36.4)* |
| NK cells | CD8αβ | 1.7 (0.9, 2.5)*,† | 1.7 (0.5, 3.5)* | 0.9 (0.6, 1.3)† |
| TCRγδ+ T cells | CD8α | 26.0 (23.0, 34.5) | 43.0 (34.7, 52.0)* | 41.9 (24.7, 55.0)* |
| TCRγδ+ T cells | CD8αβ | 4.2 (1.8, 6.4) | 17.6 (12.2, 27.5)* | 13.2 (9.7, 24.5)* |
| CD4+ T cells | CD8α | 1.5 (0.9, 2.0) | 3.8 (2.4, 6.8)* | 4.9 (3.0, 8.0)* |
| CD4+ T cells | CD8αβ | 0.6 (0.4, 0.8) | 1.8 (1.2, 3.0) | 3.5 (2.2, 5.3) |
| Gated Cells . | Assessed for Expression of: . | HIV-1 Seronegative (n = 21) . | HIV-1 Seropositive >200 CD4+cells/μL (n = 58) . | HIV-1 Seropositive <200 CD4+ cells/μL (n = 45) . |
|---|---|---|---|---|
| Median % of CD8α+ or CD8αβ+ cell subset within gated cells (25%, 75%) . | ||||
| CD8α+ T cells | CD8αβ | 90.8 (81.1, 92.6) | 96.9 (94.8, 98.0)* | 97.6 (95.3, 98.3)* |
| CD8αbright T cells | CD8αβ | 97.1 (94.7, 98.3) | 99.4 (98.6, 99.6)* | 99.2 (98.6, 99.6)* |
| NK cells | CD8α | 46.7 (41.1, 60.7) | 28.2 (23.2, 35.3)* | 25.2 (19.2, 36.4)* |
| NK cells | CD8αβ | 1.7 (0.9, 2.5)*,† | 1.7 (0.5, 3.5)* | 0.9 (0.6, 1.3)† |
| TCRγδ+ T cells | CD8α | 26.0 (23.0, 34.5) | 43.0 (34.7, 52.0)* | 41.9 (24.7, 55.0)* |
| TCRγδ+ T cells | CD8αβ | 4.2 (1.8, 6.4) | 17.6 (12.2, 27.5)* | 13.2 (9.7, 24.5)* |
| CD4+ T cells | CD8α | 1.5 (0.9, 2.0) | 3.8 (2.4, 6.8)* | 4.9 (3.0, 8.0)* |
| CD4+ T cells | CD8αβ | 0.6 (0.4, 0.8) | 1.8 (1.2, 3.0) | 3.5 (2.2, 5.3) |
All results are expressed as median % (25th percentile, 75th percentile). Statistical differences (P < .05) among the three blood donor groups per row were first analyzed by the Kruskal-Wallis test. If the results were found to be significant, each of the three data groups per row were analyzed by the Dunn test. CD8α or CD8αβ expression was assessed on gated cells defined as: CD8α+ T cells (CD8α+CD3+), NK cells (CD56+CD3−), TCRγδ+ T cells (TCRγδ+CD3+) and CD4+ T cells (CD4+CD3+). CD8αbright T cells were defined using a subjective tight gate around the main subpopulation of CD8α+ T cells, which express CD8α in high density as described.32 33
The differences between the two groups were NOT significant (P < .01) using the Dunn test.
The differences between the two groups were NOT significant (P < .01) using the Dunn test. All other data groups per row were significantly different.