Influence of IL-7 Deprivation on the Development of Thymic DC
| . | Total Cells/Lobe (×10−4) . | Dendritic Cells . | |
|---|---|---|---|
| % . | Absolute No. (×10−2) . | ||
| Control | 10.6 ± 1.2 | 0.7 | 7.4 ± 1.0 |
| Anti–IL-7 | 3.5 ± 0.7* | 0.4 | 1.6 ± 0.3* |
| . | Total Cells/Lobe (×10−4) . | Dendritic Cells . | |
|---|---|---|---|
| % . | Absolute No. (×10−2) . | ||
| Control | 10.6 ± 1.2 | 0.7 | 7.4 ± 1.0 |
| Anti–IL-7 | 3.5 ± 0.7* | 0.4 | 1.6 ± 0.3* |
Thymic lobes from 14-day-old mouse embryos were organ-cultured in the presence of neutralizing anti–IL-7 MoAb. Cells from thymic lobes were recovered after a 12-day culture period, counted, cytospun onto slides, and stained with anti–MHC class II MoAb. Percentages of MHC class II+ cells exhibiting DC morphology were obtained scoring 5,000 cells in 5 to 7 cytospin preparations. The results represent means ± SEM of four independent experiments.
Statistical significance differences between control and IL-7-treated FTOC: *P ≤ .001.