Lineage Expression by Engrafted Donor (Ly-5.1) Cells in Individual Mice at 5 Months Posttransplantation
| Cytokines . | Mice . | % B220+ Cells . | % Thy-1.2+ Cells . | % Gr-1+, Mac-1+ Cells . | % Total . | Myeloid/Lymphoid Ratio . |
|---|---|---|---|---|---|---|
| No culture | 1 | 4.8 | 3.1 | 2.6 | 10.5 | 0.33 |
| 2 | 35.5 | 22.7 | 14.7 | 72.9 | 0.25 | |
| 3 | 33.5 | 20.5 | 19.6 | 73.6 | 0.36 | |
| 4 | 33.4 | 21.2 | 13.5 | 68.1 | 0.25 | |
| 5 | 8.4 | 6.2 | 2.4 | 17.0 | 0.16 | |
| 6 | 24.9 | 18.5 | 19.9 | 63.3 | 0.46 | |
| 7 | 20.0 | 17.5 | 6.5 | 44.0 | 0.17 | |
| 8 | 23.3 | 22.8 | 8.6 | 54.7 | 0.19 | |
| 9 | 10.0 | 8.5 | 1.2 | 19.7 | 0.06 | |
| 10 | 32.7 | 27.3 | 7.5 | 67.5 | 0.13 | |
| Mean ± SD | 49.1 ± 24.7 | 0.24 ± 0.12* | ||||
| TPO | 1 | 1.9 | 1.4 | 1.1 | 4.4 | 0.33 |
| 2 | 35.9 | 26.7 | 22.6 | 85.2 | 0.36 | |
| 3 | 1.7 | 1.5 | 1.2 | 4.4 | 0.38 | |
| 4 | 13.8 | 9.0 | 5.6 | 28.4 | 0.25 | |
| 5 | 3.5 | 2.4 | 1.5 | 7.4 | 0.25 | |
| 6 | 5.8 | 3.9 | 2.2 | 11.9 | 0.23 | |
| 7† | 0.9 | |||||
| 8 | 22.7 | 17.6 | 10.4 | 50.7 | 0.26 | |
| Mean ± SD | 24.2 ± 29.8 | 0.29 ± 0.06* | ||||
| TPO, FL | 1 | 0.9 | 0.6 | 0.8 | 2.3 | 0.53 |
| 2 | 0.9 | 0.6 | 0.7 | 2.2 | 0.47 | |
| 3 | 27.5 | 25.5 | 5.0 | 58.0 | 0.09 | |
| 4 | 1.4 | 1.1 | 1.1 | 3.6 | 0.44 | |
| 5 | 20.4 | 20.4 | 12.1 | 36.5 | 0.30 | |
| 6 | 32.5 | 19.8 | 15.2 | 67.5 | 0.29 | |
| 7† | 0.9 | |||||
| 8 | 3.8 | 2.4 | 1.4 | 7.6 | 0.23 | |
| 9 | 2.9 | 1.4 | 1.3 | 5.6 | 0.30 | |
| Mean ± SD | 20.4 ± 26.4 | 0.33 ± 0.14* | ||||
| IL-6, FL | 1 | 19.2 | 8.6 | 6.5 | 34.3 | 0.23 |
| 2 | 17.9 | 12.4 | 8.5 | 38.8 | 0.28 | |
| 3 | 37.3 | 32.2 | 22.7 | 92.2 | 0.33 | |
| 4 | 6.4 | 3.0 | 3.3 | 12.7 | 0.35 | |
| 5 | 18.0 | 8.6 | 7.9 | 34.5 | 0.30 | |
| 6 | 17.4 | 10.1 | 3.9 | 31.4 | 0.14 | |
| 7 | 7.3 | 4.7 | 1.3 | 13.3 | 0.11 | |
| 8 | 3.2 | 1.0 | 1.1 | 5.3 | 0.26 | |
| 9 | 15.5 | 14.9 | 2.5 | 32.9 | 0.08 | |
| Mean ± SD | 32.8 ± 25.3 | 0.23 ± 0.10* |
| Cytokines . | Mice . | % B220+ Cells . | % Thy-1.2+ Cells . | % Gr-1+, Mac-1+ Cells . | % Total . | Myeloid/Lymphoid Ratio . |
|---|---|---|---|---|---|---|
| No culture | 1 | 4.8 | 3.1 | 2.6 | 10.5 | 0.33 |
| 2 | 35.5 | 22.7 | 14.7 | 72.9 | 0.25 | |
| 3 | 33.5 | 20.5 | 19.6 | 73.6 | 0.36 | |
| 4 | 33.4 | 21.2 | 13.5 | 68.1 | 0.25 | |
| 5 | 8.4 | 6.2 | 2.4 | 17.0 | 0.16 | |
| 6 | 24.9 | 18.5 | 19.9 | 63.3 | 0.46 | |
| 7 | 20.0 | 17.5 | 6.5 | 44.0 | 0.17 | |
| 8 | 23.3 | 22.8 | 8.6 | 54.7 | 0.19 | |
| 9 | 10.0 | 8.5 | 1.2 | 19.7 | 0.06 | |
| 10 | 32.7 | 27.3 | 7.5 | 67.5 | 0.13 | |
| Mean ± SD | 49.1 ± 24.7 | 0.24 ± 0.12* | ||||
| TPO | 1 | 1.9 | 1.4 | 1.1 | 4.4 | 0.33 |
| 2 | 35.9 | 26.7 | 22.6 | 85.2 | 0.36 | |
| 3 | 1.7 | 1.5 | 1.2 | 4.4 | 0.38 | |
| 4 | 13.8 | 9.0 | 5.6 | 28.4 | 0.25 | |
| 5 | 3.5 | 2.4 | 1.5 | 7.4 | 0.25 | |
| 6 | 5.8 | 3.9 | 2.2 | 11.9 | 0.23 | |
| 7† | 0.9 | |||||
| 8 | 22.7 | 17.6 | 10.4 | 50.7 | 0.26 | |
| Mean ± SD | 24.2 ± 29.8 | 0.29 ± 0.06* | ||||
| TPO, FL | 1 | 0.9 | 0.6 | 0.8 | 2.3 | 0.53 |
| 2 | 0.9 | 0.6 | 0.7 | 2.2 | 0.47 | |
| 3 | 27.5 | 25.5 | 5.0 | 58.0 | 0.09 | |
| 4 | 1.4 | 1.1 | 1.1 | 3.6 | 0.44 | |
| 5 | 20.4 | 20.4 | 12.1 | 36.5 | 0.30 | |
| 6 | 32.5 | 19.8 | 15.2 | 67.5 | 0.29 | |
| 7† | 0.9 | |||||
| 8 | 3.8 | 2.4 | 1.4 | 7.6 | 0.23 | |
| 9 | 2.9 | 1.4 | 1.3 | 5.6 | 0.30 | |
| Mean ± SD | 20.4 ± 26.4 | 0.33 ± 0.14* | ||||
| IL-6, FL | 1 | 19.2 | 8.6 | 6.5 | 34.3 | 0.23 |
| 2 | 17.9 | 12.4 | 8.5 | 38.8 | 0.28 | |
| 3 | 37.3 | 32.2 | 22.7 | 92.2 | 0.33 | |
| 4 | 6.4 | 3.0 | 3.3 | 12.7 | 0.35 | |
| 5 | 18.0 | 8.6 | 7.9 | 34.5 | 0.30 | |
| 6 | 17.4 | 10.1 | 3.9 | 31.4 | 0.14 | |
| 7 | 7.3 | 4.7 | 1.3 | 13.3 | 0.11 | |
| 8 | 3.2 | 1.0 | 1.1 | 5.3 | 0.26 | |
| 9 | 15.5 | 14.9 | 2.5 | 32.9 | 0.08 | |
| Mean ± SD | 32.8 ± 25.3 | 0.23 ± 0.10* |
Lineage expression of the engrafted donor cells was analyzed at 5 months posttransplantation. Background (peripheral blood of nontransplanted male mice) staining by anti–Ly-5.1 MoAb (A20-1.7) was less than 1.0%. Because the donor cells in the recipient mice that had been transplanted with cells cultured with FL or IL-6 alone showed less than 1.0%, analysis of lineage phenotypes was not performed.
There were no significant differences of the myeloid (Gr-1+, Mac-1+ cells)/lymphoid (B220+ cells and Thy-1.2+ cells) ratios among the experimental groups by unpaired t-test.
Because no donor cells were detected in these animals, analysis of lineage phenotypes was not performed.