Survival of Total Cells and Progenitors in Suspension Culture
| Cytokines in Primary Culture . | Cells . | Total CFU . | CFU-GEMM . | CFU-Meg . |
|---|---|---|---|---|
| No culture* | 1,000 | 450 | 145 | 50 |
| Medium | 0 | 0 | 0 | 0 |
| TPO | ||||
| 100 ng/mL | 356 | 203 | 68 | 15 |
| 1,000 ng/mL | 400 | 220 | 53 | 13 |
| FL | ||||
| 100 ng/mL | 222 | 0 | 0 | 0 |
| 1,000 ng/mL | 200 | 0 | 0 | 0 |
| IL-6 | ||||
| 100 ng/mL | 200 | 6 | 1 | 1 |
| 1,000 ng/mL | 178 | 5 | 1 | 1 |
| TPO, FL | 567 | 256 | 50 | 14 |
| IL-6, FL | 4,267 | 2,048 | 171 | 11 |
| Cytokines in Primary Culture . | Cells . | Total CFU . | CFU-GEMM . | CFU-Meg . |
|---|---|---|---|---|
| No culture* | 1,000 | 450 | 145 | 50 |
| Medium | 0 | 0 | 0 | 0 |
| TPO | ||||
| 100 ng/mL | 356 | 203 | 68 | 15 |
| 1,000 ng/mL | 400 | 220 | 53 | 13 |
| FL | ||||
| 100 ng/mL | 222 | 0 | 0 | 0 |
| 1,000 ng/mL | 200 | 0 | 0 | 0 |
| IL-6 | ||||
| 100 ng/mL | 200 | 6 | 1 | 1 |
| 1,000 ng/mL | 178 | 5 | 1 | 1 |
| TPO, FL | 567 | 256 | 50 | 14 |
| IL-6, FL | 4,267 | 2,048 | 171 | 11 |
One thousand Lin−Sca-1+c-kit+ cells were cultured in the presence of designated cytokines. On day 7 of culture, cells were analyzed for total CFU, CFU-GEMM, and CFU-Meg in methylcellulose culture in the presence of SF, IL-3, IL-6, FL, TPO, and EPO.
Freshly sorted 1,000 Lin−Sca-1+c-kit+ cells.