Table 1. In Vitro Colony Formation... | American Society of Hematology

Table 1.

In Vitro Colony Formation

Cytokines	No. of Colonies
Cytokines	GM	GEMM	GMM	GEM	Meg	Total
None	0	0		0	0	0
TPO
100 ng/mL	0	0	0	0	0 ± 1	0 ± 1
1,000 ng/mL	0	0	0	0	0 ± 1	0 ± 1
FL
100 ng/mL	0	0	0	0	0	0
1,000 ng/mL	0	0	0	0	0	0
IL-6
100 ng/mL	0	0	0	0	0	0
1,000 ng/mL	0	0	0	0	0	0
TPO, FL	0	0	0	0	1 ± 1	1 ± 1
IL-6, FL						10 ± 2^-150
Cocktail^-151	24 ± 4	13 ± 2	2 ± 1	0 ± 1	5 ± 2	44 ± 8

Cytokines	No. of Colonies
Cytokines	GM	GEMM	GMM	GEM	Meg	Total
None	0	0		0	0	0
TPO
100 ng/mL	0	0	0	0	0 ± 1	0 ± 1
1,000 ng/mL	0	0	0	0	0 ± 1	0 ± 1
FL
100 ng/mL	0	0	0	0	0	0
1,000 ng/mL	0	0	0	0	0	0
IL-6
100 ng/mL	0	0	0	0	0	0
1,000 ng/mL	0	0	0	0	0	0
TPO, FL	0	0	0	0	1 ± 1	1 ± 1
IL-6, FL						10 ± 2^-150
Cocktail^-151	24 ± 4	13 ± 2	2 ± 1	0 ± 1	5 ± 2	44 ± 8

One hundred Lin⁻Sca-1⁺c-kit⁺cells prepared from the marrow of 5-FU–treated mice were plated per dish in the presence of designated cytokine(s). Colonies were scored on day 14 of culture. Data represent the mean and standard deviation of values obtained from quadruplicate dishes.

F0-150

In situ identification of colony types was not possible because of the persistence of blast-like cells.

F0-151

SF, IL-3, IL-6, FL, TPO, and EPO.

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