Table 4. Degree of Mismatching of... | American Society of Hematology

Table 4.

Degree of Mismatching of Forward and Reverse Primers Included in Primer Mix 118 With the HLA Alleles Used for Specificity Determination

DNA Sample	HLA-DR Allele	Forward Primer			Reverse Primer			Amplified by DR17 Primers
DNA Sample	HLA-DR Allele	Matched	No. of Mismatches	3′ mm	Matched	No. of Mismatches	3′ mm	Amplified by DR17 Primers
C	DRB1^3-1500101	No	1	Yes	No	2	Yes	√
D	DRB1^3-1501201	No	1	Yes	Yes	0	No	√
	DRB3^3-1500201/4	No	1	Yes	Yes	0	No	√
E	DRB1^3-1500102	No	1	Yes	Yes	0	No	√
	DRB1^3-1501104	No	1	Yes	Yes	0	No	√
	DRB3^3-1500202/3	No	1	Yes	No	2	Yes	x
F	DRB1^3-1500401	No	1	Yes	No	2	Yes	x
	DRB1^3-1500701	No	2	Yes	No	2	Yes	x
	DRB4^3-1500101	No	3	No	Yes	0	No	x
	DRB7^3-1500101	Yes	0^3-150	No	No	1	No	√
G	DRB1^3-1501501	No	1	Yes	Yes	0	No	√
	DRB1^3-1500701	No	2	Yes	No	2	Yes	x
	DRB5^3-15001	No	1	Yes	No	2	Yes	x
	DRB4^3-1500101	No	3	No	Yes	0	No	x
	DRB7^3-1500101	Yes	0^3-150	No	No	1	No	√

DNA Sample	HLA-DR Allele	Forward Primer			Reverse Primer			Amplified by DR17 Primers
DNA Sample	HLA-DR Allele	Matched	No. of Mismatches	3′ mm	Matched	No. of Mismatches	3′ mm	Amplified by DR17 Primers
C	DRB1^3-1500101	No	1	Yes	No	2	Yes	√
D	DRB1^3-1501201	No	1	Yes	Yes	0	No	√
	DRB3^3-1500201/4	No	1	Yes	Yes	0	No	√
E	DRB1^3-1500102	No	1	Yes	Yes	0	No	√
	DRB1^3-1501104	No	1	Yes	Yes	0	No	√
	DRB3^3-1500202/3	No	1	Yes	No	2	Yes	x
F	DRB1^3-1500401	No	1	Yes	No	2	Yes	x
	DRB1^3-1500701	No	2	Yes	No	2	Yes	x
	DRB4^3-1500101	No	3	No	Yes	0	No	x
	DRB7^3-1500101	Yes	0^3-150	No	No	1	No	√
G	DRB1^3-1501501	No	1	Yes	Yes	0	No	√
	DRB1^3-1500701	No	2	Yes	No	2	Yes	x
	DRB5^3-15001	No	1	Yes	No	2	Yes	x
	DRB4^3-1500101	No	3	No	Yes	0	No	x
	DRB7^3-1500101	Yes	0^3-150	No	No	1	No	√

The right hand column (√) and shading indicates alleles which were amplified by this primer mix.

There are two bases unknown in the sequence of DRB7*0101, which lie in the forward primer of mix 118.