Primer Mix Composition for PCR-SSP Typing
| PM . | Primers . | Size (bp) . | Locus . | HLA Alleles Amplified . |
|---|---|---|---|---|
| 113 | 36 + 39 | 194 | DRB1 | *0101, *01021/2, *0104 |
| 114 | 36 + 40 | 195 | DRB1 | *0103 |
| 115 | 41 + 252 | 206 | DRB1 | *15011/2, *15021/2, *1503-6 |
| 185 | 41 + 491 | 136 | DRB1 | *16011/2, *16021/2,*1603-8 |
| 162 | 68 + 255 | 211 | DRB1 | *03011/2,*03021/2, *0303-8, *1107 |
| 118 | 46 + 38 | 216 | DRB1 | *03011/2, *0304/6/8/11, *1327 |
| 119 | 44 + 37 | 188 | DRB1 | *03021/2, *0305, *1109/20, *1302/5/26/29/31,*1402/3/9/13/19/24/27/30, *1608, DRB3*0206 |
| 120 | 47 + 37 + 38 | 259 | DRB1 | *0401-22, *0424, *1122,*1410 |
| 121 | 48 + 49 | 231 | DRB1 | *0701 |
| 122 | 51 | 213 | DRB1 | *0801-4, *0806-13, *0815-17,*1415 |
| 227 | 46 + 49 | 192 | DRB1 | *09012 |
| 124 | 53 + 37 | 203 | DRB1 | *1001 |
| 125 | 68 + 273 + 54 | 175 | DRB1 | *1101-29, *0308, *1204,*1411 |
| 126 | 50 + 256 | 162 | DRB1 | *1201-5 |
| 127 | 263 + 252 + 259 | 152 | DRB1 | *1301/2/8/9/16/20/27-29/31/32, *1116/20, *1416 |
| 183 | 68 + 273 + 259 | 210 | DRB1 | *1301/2/4/8/15-17/19/20/22-24/27/28/31/32, *1102/3/11/14/16/20/21,*1416 |
| 170 | 68 + 102 | 170 | DRB1 | *1303/4/12/13/21/30/32, *1413 |
| 169 | 44 + 258 | 136 | DRB1 | *1305/6, *1109 |
| 171 | 263 + 264 + 51 + 58 | 165 | DRB1 | *1401/3-5/7-8/10-12/14-15/18/23/26-28, *0809,*1117, *1318 |
| 135 | 44 + 39 + 51 | 128/151 | DRB1 | *1402/3/6/9/12/13/17/27/29/30, *1318 |
| 134 | 68 + 107 | 171 | DRB1 | *1401/7/16/22/25/26 |
| 136 | 150 + 151 | 179 | DRB3 | *0101-2, *0201-6, *0301 |
| 186 | 61 + 492 | 172 | DRB5 | *0101-5, *0202-4 |
| 140 | 283 + 314 | 151 | DRB4 | *0101-5, *0201N |
| PM . | Primers . | Size (bp) . | Locus . | HLA Alleles Amplified . |
|---|---|---|---|---|
| 113 | 36 + 39 | 194 | DRB1 | *0101, *01021/2, *0104 |
| 114 | 36 + 40 | 195 | DRB1 | *0103 |
| 115 | 41 + 252 | 206 | DRB1 | *15011/2, *15021/2, *1503-6 |
| 185 | 41 + 491 | 136 | DRB1 | *16011/2, *16021/2,*1603-8 |
| 162 | 68 + 255 | 211 | DRB1 | *03011/2,*03021/2, *0303-8, *1107 |
| 118 | 46 + 38 | 216 | DRB1 | *03011/2, *0304/6/8/11, *1327 |
| 119 | 44 + 37 | 188 | DRB1 | *03021/2, *0305, *1109/20, *1302/5/26/29/31,*1402/3/9/13/19/24/27/30, *1608, DRB3*0206 |
| 120 | 47 + 37 + 38 | 259 | DRB1 | *0401-22, *0424, *1122,*1410 |
| 121 | 48 + 49 | 231 | DRB1 | *0701 |
| 122 | 51 | 213 | DRB1 | *0801-4, *0806-13, *0815-17,*1415 |
| 227 | 46 + 49 | 192 | DRB1 | *09012 |
| 124 | 53 + 37 | 203 | DRB1 | *1001 |
| 125 | 68 + 273 + 54 | 175 | DRB1 | *1101-29, *0308, *1204,*1411 |
| 126 | 50 + 256 | 162 | DRB1 | *1201-5 |
| 127 | 263 + 252 + 259 | 152 | DRB1 | *1301/2/8/9/16/20/27-29/31/32, *1116/20, *1416 |
| 183 | 68 + 273 + 259 | 210 | DRB1 | *1301/2/4/8/15-17/19/20/22-24/27/28/31/32, *1102/3/11/14/16/20/21,*1416 |
| 170 | 68 + 102 | 170 | DRB1 | *1303/4/12/13/21/30/32, *1413 |
| 169 | 44 + 258 | 136 | DRB1 | *1305/6, *1109 |
| 171 | 263 + 264 + 51 + 58 | 165 | DRB1 | *1401/3-5/7-8/10-12/14-15/18/23/26-28, *0809,*1117, *1318 |
| 135 | 44 + 39 + 51 | 128/151 | DRB1 | *1402/3/6/9/12/13/17/27/29/30, *1318 |
| 134 | 68 + 107 | 171 | DRB1 | *1401/7/16/22/25/26 |
| 136 | 150 + 151 | 179 | DRB3 | *0101-2, *0201-6, *0301 |
| 186 | 61 + 492 | 172 | DRB5 | *0101-5, *0202-4 |
| 140 | 283 + 314 | 151 | DRB4 | *0101-5, *0201N |
All primer mixes have been previously described in Bunce et al29 with the exception of primer mix 136. Primer mix 136 consists of primer 150 (5′-CCACgTTTCTTggAgCTgC) and primer 151 (5′-CCgTAgTTgTgTCTgCAgTAA), which amplify all DRB3 alleles (PM, primer mix number; Size, size of PCR product).