Effect of Anti-fas and Dexamethasone on Mutant c-jun–Containing MM Cells
| Cell Line . | Drug . | % Viability . | % DNA Fragmentation . |
|---|---|---|---|
| neo control | None-control | 81 ± 5 | 6.4 ± 1 |
| AF, 0.1 μg/mL | 67 ± 4* | 16.4 ± 2* | |
| AF, 0.25 μg/mL | 60 ± 4* | 22 ± 3* | |
| AF, 0.5 μg/mL | 35 ± 2* | 29.7 ± 3* | |
| dom neg | None-control | 83 ± 5 | 3.1 ± 1 |
| AF, 0.1 μg/mL | 82 ± 5 | 4.4 ± 1 | |
| AF, 0.25 μg/mL | 80 ± 3 | 3.5 ± 1 | |
| AF, 0.5 μg/mL | 81 ± 4 | 4.2 ± 1 | |
| neo control | Non-control | 92 ± 4 | 3.9 ± 1 |
| Dex, 10−6 mol/L | 73 ± 3 | 21 ± 3 | |
| Dex, 2 × 10−6mol/L | 61 ± 5 | 29 ± 3 | |
| dom neg | None-control | 90 ± 6 | 4.1 ± 1 |
| Dex, 10−6 mol/L | 54 ± 5† | 33.8 ± 3† | |
| Dex, 2 × 10−6 mol/L | 38 ± 4† | 44.2 ± 4† |
| Cell Line . | Drug . | % Viability . | % DNA Fragmentation . |
|---|---|---|---|
| neo control | None-control | 81 ± 5 | 6.4 ± 1 |
| AF, 0.1 μg/mL | 67 ± 4* | 16.4 ± 2* | |
| AF, 0.25 μg/mL | 60 ± 4* | 22 ± 3* | |
| AF, 0.5 μg/mL | 35 ± 2* | 29.7 ± 3* | |
| dom neg | None-control | 83 ± 5 | 3.1 ± 1 |
| AF, 0.1 μg/mL | 82 ± 5 | 4.4 ± 1 | |
| AF, 0.25 μg/mL | 80 ± 3 | 3.5 ± 1 | |
| AF, 0.5 μg/mL | 81 ± 4 | 4.2 ± 1 | |
| neo control | Non-control | 92 ± 4 | 3.9 ± 1 |
| Dex, 10−6 mol/L | 73 ± 3 | 21 ± 3 | |
| Dex, 2 × 10−6mol/L | 61 ± 5 | 29 ± 3 | |
| dom neg | None-control | 90 ± 6 | 4.1 ± 1 |
| Dex, 10−6 mol/L | 54 ± 5† | 33.8 ± 3† | |
| Dex, 2 × 10−6 mol/L | 38 ± 4† | 44.2 ± 4† |
Neo control or c-jun dominant negative–containing 8226 cells cultured with anti-fas (AF) for 20 hours or dexamethasone (Dex) for 48 hours and viability and DNA fragmentation (diphenylamine assay) was evaluated. Data are means ± SD of three separate experiments.
Significantly different (P < .05) from corresponding control (culture without drug).
Significantly different (P < .05) from corresponding values in neo-control cells.