Characterization of Expanded Cells
| Expansion rate | 119.9-fold ± 48.6-fold |
| Viability | 96.4% ± 2.7% |
| Purity of erythroid cells | 91.4% ± 5.9% |
| Purity of ECFC | |
| CSF combination | |
| EP | 40.5% ± 4.3% |
| EP + IL-3 | 41.1% ± 3.3% |
| EP + IL-3 + SCF | 42.5% ± 4.0% |
| Surface Marker | |
| Gly A | 79.5% ± 6.1% |
| CD34 | 2.6% ± 1.0% |
| CD117 | 33.0% ± 15.6% |
| CD13 | 3.7% ± 1.1% |
| Expansion rate | 119.9-fold ± 48.6-fold |
| Viability | 96.4% ± 2.7% |
| Purity of erythroid cells | 91.4% ± 5.9% |
| Purity of ECFC | |
| CSF combination | |
| EP | 40.5% ± 4.3% |
| EP + IL-3 | 41.1% ± 3.3% |
| EP + IL-3 + SCF | 42.5% ± 4.0% |
| Surface Marker | |
| Gly A | 79.5% ± 6.1% |
| CD34 | 2.6% ± 1.0% |
| CD117 | 33.0% ± 15.6% |
| CD13 | 3.7% ± 1.1% |
Values are mean ± standard deviation of eight independent experiments.
Abbreviations: ECFC, erythroid colony-forming cells; CSF, colony-stimulating factor; EP, erythropoietin; IL-3, interleukin-3; SCF, stem cell factor.