Differential Cell Growth Inhibition (IC50) of 5-FC and 5-FU in Primary Normal HMECs and in BCC Lines, MCF-7 and MDA-MB-453, Before or After Incubation With the Ad.CMV-CD Vector
| . | Inhibitory Concentration50 (IC50) After Incubation With . | ||
|---|---|---|---|
| 5-FC (μmol/L)* Alone . | 5-FU (μmol/L)* Alone . | 5-FC (μmol/L) + (Ad.CMV-CD) Combined . | |
| HMEC | 3,000 | 1 ± 0.5 | 10 (10 MOI)-151 |
| MDA-MD-453 | >10,000 | 30 ± 5 | 100 (50 MOI)-151 |
| MCF-7 | >10,000 | 4 ± 1 | 10 (10 MOI)-151 |
| CD34 + 0.1% MCF-7 | — | — | 10 (10 MOI)-152 |
| . | Inhibitory Concentration50 (IC50) After Incubation With . | ||
|---|---|---|---|
| 5-FC (μmol/L)* Alone . | 5-FU (μmol/L)* Alone . | 5-FC (μmol/L) + (Ad.CMV-CD) Combined . | |
| HMEC | 3,000 | 1 ± 0.5 | 10 (10 MOI)-151 |
| MDA-MD-453 | >10,000 | 30 ± 5 | 100 (50 MOI)-151 |
| MCF-7 | >10,000 | 4 ± 1 | 10 (10 MOI)-151 |
| CD34 + 0.1% MCF-7 | — | — | 10 (10 MOI)-152 |
*Cells (10,000/well) were plated in the above-noted incubations in triplicate in 96-well plates. Twenty-four hours later, different drug concentrations (see above) were added to the medium and cell proliferation was assessed after 5 days using a colorimetric assay as reported in the Materials and Methods.
Cells were exposed to Ad.CMV-CD for 90 minutes in serum-free medium at different MOI, and then 10,000 cells/well were plated in triplicate in 96-well plates. Twenty-four hours later, 5-FC and 5-FU were added to the medium and cell proliferation was determined after 5 days by a colorimetric assay.
Each of 9 independent wells was seeded with 10,000 cells/well for each condition in the limiting dilution assay. Dilution refers to the total number of cells reseeded.