Histomorphology and Phenotype of Bone Marrow Myeloid Cells
| Source (age) . | No. . | Cellularity-150 . | Differential Cell Counts (%) . | |||
|---|---|---|---|---|---|---|
| Blasts and Promyelocytes . | Myelocytes and Metamyelocytes . | Bands and Polys . | Erythroid and Lymphoid . | |||
| SPF IL-2+/+ (6 wk) | 6 | 1.61 ± 0.3 | 5 ± 1 | 15 ± 5 | 43 ± 11 | 35 ± 5 |
| SPF IL-2−/− (6 wk) | 5 | 1.03 ± 0.1-151 | 17 ± 7-152 | 11 ± 4 | 18 ± 7-153 | 44 ± 10 |
| Gnotobiotic IL-2+/+ (4 wk) | 5 | 1.33 ± 0.4 | 6 ± 2 | 19 ± 7 | 40 ± 12 | 35 ± 8 |
| Gnotobiotic IL-2−/− (4 wk) | 4 | 1.45 ± 0.9 | 9 ± 3 | 14 ± 4 | 37 ± 6 | 39 ± 13 |
| Gnotobiotic IL-2+/+ (7 wk) | 4 | 1.73 ± 0.4 | 7 ± 2 | 18 ± 4 | 40 ± 9 | 33 ± 6 |
| Gnotobiotic IL-2−/− (7 wk) | 6 | 1.00 ± 0.2-151 | 22 ± 5-152 | 12 ± 5 | 16 ± 8-153 | 49 ± 14 |
| B.6 + IL-2+/+ T cells-155 | 5 | 1.74 ± 0.5 | 4 ± 2 | 15 ± 4 | 44 ± 13 | 34 ± 4 |
| B.6 + IL-2−/− T cells-155 | 5 | 1.66 ± 0.3 | 5 ± 3 | 18 ± 6 | 39 ± 9 | 39 ± 8 |
| Source (age) . | No. . | Cellularity-150 . | Differential Cell Counts (%) . | |||
|---|---|---|---|---|---|---|
| Blasts and Promyelocytes . | Myelocytes and Metamyelocytes . | Bands and Polys . | Erythroid and Lymphoid . | |||
| SPF IL-2+/+ (6 wk) | 6 | 1.61 ± 0.3 | 5 ± 1 | 15 ± 5 | 43 ± 11 | 35 ± 5 |
| SPF IL-2−/− (6 wk) | 5 | 1.03 ± 0.1-151 | 17 ± 7-152 | 11 ± 4 | 18 ± 7-153 | 44 ± 10 |
| Gnotobiotic IL-2+/+ (4 wk) | 5 | 1.33 ± 0.4 | 6 ± 2 | 19 ± 7 | 40 ± 12 | 35 ± 8 |
| Gnotobiotic IL-2−/− (4 wk) | 4 | 1.45 ± 0.9 | 9 ± 3 | 14 ± 4 | 37 ± 6 | 39 ± 13 |
| Gnotobiotic IL-2+/+ (7 wk) | 4 | 1.73 ± 0.4 | 7 ± 2 | 18 ± 4 | 40 ± 9 | 33 ± 6 |
| Gnotobiotic IL-2−/− (7 wk) | 6 | 1.00 ± 0.2-151 | 22 ± 5-152 | 12 ± 5 | 16 ± 8-153 | 49 ± 14 |
| B.6 + IL-2+/+ T cells-155 | 5 | 1.74 ± 0.5 | 4 ± 2 | 15 ± 4 | 44 ± 13 | 34 ± 4 |
| B.6 + IL-2−/− T cells-155 | 5 | 1.66 ± 0.3 | 5 ± 3 | 18 ± 6 | 39 ± 9 | 39 ± 8 |
Differential counts represent the percentage of 300 cells counted from cytocentrifuge preparations of bone marrow cells.
Marrow cellularity is cells × 107/femur.
P < .01.
P < .05.
P < .05 compared with +/+.
B.6 mice were transfused with 107IL-2−/− or IL-2+/+ lymph node T cells and 6 weeks later the cellularity and composition of host bone marrow cells determined.