Modulation of CD95 Expression and Sensitivity to CD95-Mediated Apoptosis of Lymphoma and Nontumoral B Cells by CD40L and IL-4
| Case . | CD95 Expression . | CD95-Mediated Apoptosis . | ||||||
|---|---|---|---|---|---|---|---|---|
| Medium . | IL-4 . | CD40Lig-L Cells . | CD40Lig-L Cells + IL-4 . | Medium . | IL-4 . | CD40Lig-L Cells . | CD40Lig-L Cells + IL-4 . | |
| NT-1 | 25 | 52 | 400 | 480 | ≤5 | ≤5 | 60 | 84 |
| NT-2 | 20 | 30 | 407 | 434 | NC* | ≤5 | 55 | 65 |
| LCL-3 | 17 | ND | ND | 240 | NC | ND | ND | 14 |
| MCL-3 | 3 | ND | ND | 160 | ≤5 | ≤5 | ≤5 | ≤5 |
| MCL-4 | 3 | ND | ND | 134 | NC | ≤5 | ≤5 | ≤5 |
| MCL-6 | 12 | ND | ND | 213 | NC | ND | ND | 37 |
| FL-1 | 88 | ND | ND | 237 | ≤5 | ≤5 | 15 | NC |
| FL-3 | 64 | ND | ND | 522 | ≤5 | ≤5 | 22 | 15 |
| DLCL-2 | ND | ND | ND | ND | NC | 9 | ≤5 | ≤5 |
| DLCL-3 | 39 | ND | ND | 347 | ≤5 | ≤5 | 7 | 6 |
| Case . | CD95 Expression . | CD95-Mediated Apoptosis . | ||||||
|---|---|---|---|---|---|---|---|---|
| Medium . | IL-4 . | CD40Lig-L Cells . | CD40Lig-L Cells + IL-4 . | Medium . | IL-4 . | CD40Lig-L Cells . | CD40Lig-L Cells + IL-4 . | |
| NT-1 | 25 | 52 | 400 | 480 | ≤5 | ≤5 | 60 | 84 |
| NT-2 | 20 | 30 | 407 | 434 | NC* | ≤5 | 55 | 65 |
| LCL-3 | 17 | ND | ND | 240 | NC | ND | ND | 14 |
| MCL-3 | 3 | ND | ND | 160 | ≤5 | ≤5 | ≤5 | ≤5 |
| MCL-4 | 3 | ND | ND | 134 | NC | ≤5 | ≤5 | ≤5 |
| MCL-6 | 12 | ND | ND | 213 | NC | ND | ND | 37 |
| FL-1 | 88 | ND | ND | 237 | ≤5 | ≤5 | 15 | NC |
| FL-3 | 64 | ND | ND | 522 | ≤5 | ≤5 | 22 | 15 |
| DLCL-2 | ND | ND | ND | ND | NC | 9 | ≤5 | ≤5 |
| DLCL-3 | 39 | ND | ND | 347 | ≤5 | ≤5 | 7 | 6 |
CD95 expression was measured after a 3-day culture in the indicated conditions and is expressed in MFI values. CD95-mediated apoptosis was measured after an 18-hour incubation with CD95 MoAb or IgM MoAb as control after a 3-day culture in the indicated conditions. Apoptosis is expressed as a percentage of specific apoptosis as described in Materials and Methods.
Abbreviations: ND, not done; NC, not calculated.
Percentage of apoptotic cells was not calculated when the total number of dead cells in control was greater than 60%.