Correlation Between DNA-PCR for the Derivative Chromosome 5 and Chromosome 2, RT-PCR for the Reciprocal Translocation, and Immunohistochemistry Using ALK1 and Anti-p80 Antibodies in the Cases With Amplifiable NPM-ALK Transcripts by RT-PCR
| . | Case No. . | Chromosome 5 . | IHC ALK1 . | IHC p80 . | Chromosome 2 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| RT-PCR . | Nested RT-PCR . | DNA-PCR . | Nested DNA-PCR . | RT-PCR . | Nested RT-PCR . | DNA-PCR . | Nested DNA-PCR . | ||||
| Secondary CLCL | 1 | + | + | + | + | + | + | − | − | +* | − |
| 2 | + | + | + | + | + | + | − | + | + | + | |
| 3 | + | + | + | + | + | + | − | + | + | + | |
| Lymphomatoid papulosis | 4 | − | + | − | −-151 | − | + | − | − | − | − |
| Primary CLCL | 5 | − | + | − | − | − | + | − | − | − | − |
| 6 | − | + | − | − | − | + | − | − | − | − | |
| 7 | − | + | − | +-151 | − | − | − | − | − | − | |
| 8 | + | + | + | + | + | + | − | + | + | + | |
| 9 | − | + | − | +-151 | − | + | − | − | − | − | |
| 10 | + | + | − | − | − | − | − | − | − | − | |
| 11 | − | + | − | − | − | − | − | − | − | − | |
| . | Case No. . | Chromosome 5 . | IHC ALK1 . | IHC p80 . | Chromosome 2 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| RT-PCR . | Nested RT-PCR . | DNA-PCR . | Nested DNA-PCR . | RT-PCR . | Nested RT-PCR . | DNA-PCR . | Nested DNA-PCR . | ||||
| Secondary CLCL | 1 | + | + | + | + | + | + | − | − | +* | − |
| 2 | + | + | + | + | + | + | − | + | + | + | |
| 3 | + | + | + | + | + | + | − | + | + | + | |
| Lymphomatoid papulosis | 4 | − | + | − | −-151 | − | + | − | − | − | − |
| Primary CLCL | 5 | − | + | − | − | − | + | − | − | − | − |
| 6 | − | + | − | − | − | + | − | − | − | − | |
| 7 | − | + | − | +-151 | − | − | − | − | − | − | |
| 8 | + | + | + | + | + | + | − | + | + | + | |
| 9 | − | + | − | +-151 | − | + | − | − | − | − | |
| 10 | + | + | − | − | − | − | − | − | − | − | |
| 11 | − | + | − | − | − | − | − | − | − | − | |
*Positive Southern hybridization with NPM-2P but not with ALK-2P.
Positive Southern hybridization with ALK-5P but not with NPM-5P.